Cerato-platanin (CP) is a 120 amino acids protein, secreted by the Ascomycete Ceratocystis fimbriata f. sp. platani (Cfp), the causal agent of the plane canker stain. CP is located in the Cfp mycelial cell walls, and is early secreted when Cfp is grown in axenic culture. CP elicits phytoalexin synthesis and/or cell necrosis in host and in non-host tissues. The minimum CP concentration able to restrict the 50% Cfp growth on plane leaves is of about 5 x 10-5 M; the maximum eliciting effect occurs 24-48 hours post treatment. At this time, numerous defense-related genes are over-expressed, as it has been shown by Suppressive Subtractive Hybridisation. The complete sequence of the cDNA was identified; the genomic sequence of the coding region contained an intron of 59 bp. Results so far obtained by immunotechnical experiments on a total of 17 strains indicate that a CP-homologous protein occurs in all strains of C. fimbriata and in some other species of Ceratocystis. For some strains of C. fimbriata the coding sequences of the cp-hortologous genes have been obtained, and then the sequences of the deduced proteins. Cerato-platanin, an host resistance inducing protein, is produced also by fungi other than C. fimbriata f. sp. platani
Cerato-platanin, an host resistance inducing protein, is produced also by fungi other than C. fimbriata f. sp. platani
BERNARDI, RODOLFO;
2006-01-01
Abstract
Cerato-platanin (CP) is a 120 amino acids protein, secreted by the Ascomycete Ceratocystis fimbriata f. sp. platani (Cfp), the causal agent of the plane canker stain. CP is located in the Cfp mycelial cell walls, and is early secreted when Cfp is grown in axenic culture. CP elicits phytoalexin synthesis and/or cell necrosis in host and in non-host tissues. The minimum CP concentration able to restrict the 50% Cfp growth on plane leaves is of about 5 x 10-5 M; the maximum eliciting effect occurs 24-48 hours post treatment. At this time, numerous defense-related genes are over-expressed, as it has been shown by Suppressive Subtractive Hybridisation. The complete sequence of the cDNA was identified; the genomic sequence of the coding region contained an intron of 59 bp. Results so far obtained by immunotechnical experiments on a total of 17 strains indicate that a CP-homologous protein occurs in all strains of C. fimbriata and in some other species of Ceratocystis. For some strains of C. fimbriata the coding sequences of the cp-hortologous genes have been obtained, and then the sequences of the deduced proteins. Cerato-platanin, an host resistance inducing protein, is produced also by fungi other than C. fimbriata f. sp. plataniI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
