Secondary metabolism is irnportant tbr the procluction of many grape-derived compounds that contribute to the quality of r,vine. The airn of this study was to discover genes involved in grape secondary rnetabolism pathways by stirnulating their production in grape cell suspension cultures. Among numerous elicitors tested, methyl jasmonate (MeJA) induced the production of a wide range of sesquitelpenes in Cabernet Sauvignon cell suspension cultures derived from berries. To enable significant microarray comparisons, the interactions of other elicitors alone and in combination with with MeJA were explored. We found that MeJA and jasmonic acid (JA) activate sesquitelpene synthesis, but when salicylic acid (SA) was added in addition to MeJA, sesquiterpenes were not produced. Microaray analyses on the induced cultures confirmed the results of the volatile analyses, and also indicated that genes from other secondary metabolite pathways are differentially expressed in response to the treatments. Our fìndings suggest that such inducible cell culture systems are powerful tools for discovering genes associated with secondary metabolite production.
Analysis of grape cell suspensions reveals changes in secondary metabolism in response to various elicitors
D'ONOFRIO, CLAUDIO;
2006-01-01
Abstract
Secondary metabolism is irnportant tbr the procluction of many grape-derived compounds that contribute to the quality of r,vine. The airn of this study was to discover genes involved in grape secondary rnetabolism pathways by stirnulating their production in grape cell suspension cultures. Among numerous elicitors tested, methyl jasmonate (MeJA) induced the production of a wide range of sesquitelpenes in Cabernet Sauvignon cell suspension cultures derived from berries. To enable significant microarray comparisons, the interactions of other elicitors alone and in combination with with MeJA were explored. We found that MeJA and jasmonic acid (JA) activate sesquitelpene synthesis, but when salicylic acid (SA) was added in addition to MeJA, sesquiterpenes were not produced. Microaray analyses on the induced cultures confirmed the results of the volatile analyses, and also indicated that genes from other secondary metabolite pathways are differentially expressed in response to the treatments. Our fìndings suggest that such inducible cell culture systems are powerful tools for discovering genes associated with secondary metabolite production.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
