The detrimental effects of virus infections on yield quantity/quality highlight the importance of virus-free propagation material and underline the need to produce virus-free plant material for the growth industry. Since 1992, this Unit investigates the antiviral activity of several drugs improving eradication of virus diseases as chemotherapy offers wide alternative possibilities. At the beginning our researches were focused on the use of three well known synthetic nucleotide analogues: ribavirin, DHT and DHPA. Results were obtained in the control of Plum Pox Potyvirus and Prunus Necrotic Ringspot Ilarvirus on a selection of Prunus cerasifera explants. From these successes, new antiviral groups characterized by different action mechanism were investigated: inosine monophosphate dehydrogenase (IMPDH), S-adenosilhomocysteine hydrolase (SAH) and neuraminidase (NA) inhibitors. The first screening was conducted on the in vitro system Nicotiana tabacum cv Xanthi/Cucumber Mosaic Cucumovirus treated with drugs of these groups obtaining positive results for all combinations (reported on the XIII Meeting SIPaV). Next trials were applied for the sanitation of grapevines characterized by compromised sanitary condition. Treatments showed a good control on Grapevine Leafroll associated Ampelovirus-3 infection in in vitro Sangiovese explants achiving 100% free explants for NA inhibitors. In vitro Sagrantino plantlets infected by Grapevine Vitivirus A were treated with the same compounds and virus eradication was obtained with the combination of IMPDH and SAH inhibitors.
New advances on in vitro antiviral chemotherapy
PANATTONI, ALESSANDRA;TRIOLO, ENRICO
2007-01-01
Abstract
The detrimental effects of virus infections on yield quantity/quality highlight the importance of virus-free propagation material and underline the need to produce virus-free plant material for the growth industry. Since 1992, this Unit investigates the antiviral activity of several drugs improving eradication of virus diseases as chemotherapy offers wide alternative possibilities. At the beginning our researches were focused on the use of three well known synthetic nucleotide analogues: ribavirin, DHT and DHPA. Results were obtained in the control of Plum Pox Potyvirus and Prunus Necrotic Ringspot Ilarvirus on a selection of Prunus cerasifera explants. From these successes, new antiviral groups characterized by different action mechanism were investigated: inosine monophosphate dehydrogenase (IMPDH), S-adenosilhomocysteine hydrolase (SAH) and neuraminidase (NA) inhibitors. The first screening was conducted on the in vitro system Nicotiana tabacum cv Xanthi/Cucumber Mosaic Cucumovirus treated with drugs of these groups obtaining positive results for all combinations (reported on the XIII Meeting SIPaV). Next trials were applied for the sanitation of grapevines characterized by compromised sanitary condition. Treatments showed a good control on Grapevine Leafroll associated Ampelovirus-3 infection in in vitro Sangiovese explants achiving 100% free explants for NA inhibitors. In vitro Sagrantino plantlets infected by Grapevine Vitivirus A were treated with the same compounds and virus eradication was obtained with the combination of IMPDH and SAH inhibitors.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
