Long-terminal-repeat (LTR) retrotransposons represent a large proportion of repetitive DNA in plant genome and in recent years several molecular marker methods based on retrotransposons have been developed. In grapevine genome the complete sequence of one gypsy-like retrotransposon (Gret1) and the partial sequences of two copia-like retrotransposons (Vine-1 and Tvv1) have been isolated, and some molecular markers based on the insertion polymorphisms of the above mentioned retrotransposons have been recently tested for identification of grapevine species and varieties. In this study we analysed the capacity of Inter-Retrotransposon Amplified Polymorphism (IRAP) and Retrotransposon-Microsatellite Amplified Polymorphism (REMAP) molecular markers in the discrimination of 18 Vitis genotypes: 6 Vitis species (Vitis arizonica, Vitis cinerea, Vitis labrusca, Vitis rupestrìs, Vitis rotundifolia and Vitis vinifera subsp. sylvestris) and 12 Vitis vinifera subsp. sativa varieties (“Cabernet Franc”, “Cabernet Sauvignon”, “Chasselas”, “Fiano”, “Malvasia Bianca Cenaia 2”, “Moscato bianco di Canelli”, “Pinot Nero”, “Rkatzitely”, “Sauvignon Blanc”, “Sangiovese”, “Sultanina”, “Vermentino”). The insertion polymorphisms of Gret1, Vine-1 and Tvv1 retrotransposons and the allocation of the Vitis genotypes in IRAP and REMAP phylogenetic trees are discussed.
IRAP, REMAP and S-SAP molecular markers in grapevine genotype identification and phylogenetic studies
GIORDANI, TOMMASO;D'ONOFRIO, CLAUDIO;NATALI, LUCIA;SCALABRELLI, GIANCARLO;CAVALLINI, ANDREA
2007-01-01
Abstract
Long-terminal-repeat (LTR) retrotransposons represent a large proportion of repetitive DNA in plant genome and in recent years several molecular marker methods based on retrotransposons have been developed. In grapevine genome the complete sequence of one gypsy-like retrotransposon (Gret1) and the partial sequences of two copia-like retrotransposons (Vine-1 and Tvv1) have been isolated, and some molecular markers based on the insertion polymorphisms of the above mentioned retrotransposons have been recently tested for identification of grapevine species and varieties. In this study we analysed the capacity of Inter-Retrotransposon Amplified Polymorphism (IRAP) and Retrotransposon-Microsatellite Amplified Polymorphism (REMAP) molecular markers in the discrimination of 18 Vitis genotypes: 6 Vitis species (Vitis arizonica, Vitis cinerea, Vitis labrusca, Vitis rupestrìs, Vitis rotundifolia and Vitis vinifera subsp. sylvestris) and 12 Vitis vinifera subsp. sativa varieties (“Cabernet Franc”, “Cabernet Sauvignon”, “Chasselas”, “Fiano”, “Malvasia Bianca Cenaia 2”, “Moscato bianco di Canelli”, “Pinot Nero”, “Rkatzitely”, “Sauvignon Blanc”, “Sangiovese”, “Sultanina”, “Vermentino”). The insertion polymorphisms of Gret1, Vine-1 and Tvv1 retrotransposons and the allocation of the Vitis genotypes in IRAP and REMAP phylogenetic trees are discussed.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
