The findings that grafts of adult peritoneal cells enhance the antibody response of newborn mice (1, 2), rats (3) and rabbits (4) have suggested that the immunologic immaturity of newborns might partly be due to a functional deficiency of macrophages. The results reported here show that unstimulated peritoneal cells (PC), but not peritoneal cells induced by thioglycolate (IPC), are endowed with the property of potentiating the antibody response of newborn mice to sheep red blood cells (SRBC). Inbred BALB/c mice were used throughout the experiments. PC and IPC were collected from mice of both sexes aged 8 to 12 weeks, the latter cells 3 days after i.p. inoculation of 3 ml thioglycolate medium (Difco Laboratories. Detroit, Mich.), by washing the peritoneal cavity with 5 ml of Eagle's minimal essential medium containing 5 i.u./ml of heparin. Litters of 6 to 8 mice were divided into four groups.
EFFECT OF ADULT PERITONEAL CELLS ON ANTIBODY RESPONSE OF NEWBORN MICE TO SHEEP RED BLOOD CELLS
BENDINELLI, MAURO;SENESI, SONIA;FALCONE, GIUSEPPE
1971-01-01
Abstract
The findings that grafts of adult peritoneal cells enhance the antibody response of newborn mice (1, 2), rats (3) and rabbits (4) have suggested that the immunologic immaturity of newborns might partly be due to a functional deficiency of macrophages. The results reported here show that unstimulated peritoneal cells (PC), but not peritoneal cells induced by thioglycolate (IPC), are endowed with the property of potentiating the antibody response of newborn mice to sheep red blood cells (SRBC). Inbred BALB/c mice were used throughout the experiments. PC and IPC were collected from mice of both sexes aged 8 to 12 weeks, the latter cells 3 days after i.p. inoculation of 3 ml thioglycolate medium (Difco Laboratories. Detroit, Mich.), by washing the peritoneal cavity with 5 ml of Eagle's minimal essential medium containing 5 i.u./ml of heparin. Litters of 6 to 8 mice were divided into four groups.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
