During 2010, a phytosanitary survey was conducted, concerning the occurrence of some of the main virus, viroid and phytoplasma pathogens affecting Malus and Pyrus autochthon germplasm from Tuscany. Samples, consisting of leaves, shoots and phloematic tissues, were collected during spring 2010. In detail, a total number of 97 plants, 73 of which belonging to 29 Malus varieties, and 24 belonging to 13 Pyrus varieties, were tested by RT-PCR and nested PCR for the subsequent pathogens: Candidatus Phytoplasma mali (Ca. P. mali), Candidatus Phytoplasma pyri (Ca. P. pyri), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple mosaic virus (ApMV), Apple dimple fruit viroid (ADFVd) e Apple scar skin viroid (ASSVd). Among the eight pathogens investigated, ACLSV and ASPV proved to be the most widespread (both present in 67.0% of tested samples), while ASGV was present in 14.4% of surveyed plants. In detail, ACLSV was detected on 59 Malus and 6 Pyrus plants, while ASPV was found on 54 and 13 accessions of Malus and Pyrus, respectively. Concerning ASGV, infections by this virus have been detected on 13 Malus plants and 1 of Pyrus. The remaining pathogens (ApMV, ADFVd, ASSVd, Ca. P. mali e Ca. P. pyri) were never detected in the surveyed plants
Phytosanitary survey on pome fruit autochthonous germoplasm in Tuscany
DELLA BARTOLA, MICHELE;MATERAZZI, ALBERTO
2011-01-01
Abstract
During 2010, a phytosanitary survey was conducted, concerning the occurrence of some of the main virus, viroid and phytoplasma pathogens affecting Malus and Pyrus autochthon germplasm from Tuscany. Samples, consisting of leaves, shoots and phloematic tissues, were collected during spring 2010. In detail, a total number of 97 plants, 73 of which belonging to 29 Malus varieties, and 24 belonging to 13 Pyrus varieties, were tested by RT-PCR and nested PCR for the subsequent pathogens: Candidatus Phytoplasma mali (Ca. P. mali), Candidatus Phytoplasma pyri (Ca. P. pyri), Apple chlorotic leaf spot virus (ACLSV), Apple stem pitting virus (ASPV), Apple stem grooving virus (ASGV), Apple mosaic virus (ApMV), Apple dimple fruit viroid (ADFVd) e Apple scar skin viroid (ASSVd). Among the eight pathogens investigated, ACLSV and ASPV proved to be the most widespread (both present in 67.0% of tested samples), while ASGV was present in 14.4% of surveyed plants. In detail, ACLSV was detected on 59 Malus and 6 Pyrus plants, while ASPV was found on 54 and 13 accessions of Malus and Pyrus, respectively. Concerning ASGV, infections by this virus have been detected on 13 Malus plants and 1 of Pyrus. The remaining pathogens (ApMV, ADFVd, ASSVd, Ca. P. mali e Ca. P. pyri) were never detected in the surveyed plantsI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
