Dendritic cells (DCs) represent the most important antigen presenting cells (APCs). In vivo they originate from bone marrow myeloid and lymphoid precursors. In a first stage, the so-called immature DCs stay into nonlymphoid tissues and show high capacity of antigen capture and processing, but low T cell stimulatory capacity. After the internalization of the antigen, in the presence of inflammatory mediators, DCs mature and migrate out of nonlymphoid tissues into the blood or lymph, reaching secondary lymphoid organs. In this second stage the mature DCs lose the ability to capture antigens and increase the capacity to stimulate T cells, controlling the immune responses. Indeed, DCs are a family of heterogeneous cells and each subset exerts control over a different area of immunity, activating innate and acquired systems or maintaining the tolerance to antigens. For in vitro studies, dendritic cells can be isolated directly from peripheral blood through blood dendritic cell antibodies (BDCA), but they have been difficult to obtain due to their low concentration. So a wide variety of methods to have DCs has been performed, including the generation from circling CD14+ monocytes or from CD34+ progenitors. A multitude of systems have been carried out also to obtain these precursor cells.
Generation of Dendritic Cells: A Critical Comparison of Different Methodological Approaches
LEGITIMO, ANNALISA;CONSOLINI, RITA
2011-01-01
Abstract
Dendritic cells (DCs) represent the most important antigen presenting cells (APCs). In vivo they originate from bone marrow myeloid and lymphoid precursors. In a first stage, the so-called immature DCs stay into nonlymphoid tissues and show high capacity of antigen capture and processing, but low T cell stimulatory capacity. After the internalization of the antigen, in the presence of inflammatory mediators, DCs mature and migrate out of nonlymphoid tissues into the blood or lymph, reaching secondary lymphoid organs. In this second stage the mature DCs lose the ability to capture antigens and increase the capacity to stimulate T cells, controlling the immune responses. Indeed, DCs are a family of heterogeneous cells and each subset exerts control over a different area of immunity, activating innate and acquired systems or maintaining the tolerance to antigens. For in vitro studies, dendritic cells can be isolated directly from peripheral blood through blood dendritic cell antibodies (BDCA), but they have been difficult to obtain due to their low concentration. So a wide variety of methods to have DCs has been performed, including the generation from circling CD14+ monocytes or from CD34+ progenitors. A multitude of systems have been carried out also to obtain these precursor cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
