In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.

Quantitative Photo Activated Localization Microscopy: Unraveling the Effects of Photoblinking RID D-6414-2011

SCARSELLI, MARCO
Co-primo
;
2011-01-01

Abstract

In this work we discuss how to use photophysical information for improved quantitative measurements using Photo Activated Localization Microscopy (PALM) imaging. We introduce a method that reliably estimates the number of photoblinking molecules present in a biological sample and gives a robust way to quantify proteins at the single-cell level from PALM images. We apply this method to determine the amount of β2 adrenergic receptor, a prototypical G Protein Coupled Receptor, expressed on the plasma membrane of HeLa cells.
2011
Annibale, P; Vanni, S; Scarselli, Marco; Rothlisberger, U; Radenovic, A.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/151021
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