Reversible and selective binding of a dynamically racemic europium(iii) complex to α 1-acid glycoprotein and α 1- antitrypsin is characterised by a significant change in the europium total emission spectral fingerprint and the switching on of a large circularly polarised luminescence (CPL) signal from the metal centre. Observation of an induced CD into the ligand chromophore in the presence of α 1- AGP allows a structure for the protein-bound complex to be postulated. A direct determination of elevated α 1-AGP levels in human serum was achieved by monitoring changes in the intensity ratio of Eu emission bands.
A chiral probe for the acute phase proteins alpha-1-acid glycoprotein and alpha-1-antitrypsin based on europium luminescence
DI BARI, LORENZO;
2012-01-01
Abstract
Reversible and selective binding of a dynamically racemic europium(iii) complex to α 1-acid glycoprotein and α 1- antitrypsin is characterised by a significant change in the europium total emission spectral fingerprint and the switching on of a large circularly polarised luminescence (CPL) signal from the metal centre. Observation of an induced CD into the ligand chromophore in the presence of α 1- AGP allows a structure for the protein-bound complex to be postulated. A direct determination of elevated α 1-AGP levels in human serum was achieved by monitoring changes in the intensity ratio of Eu emission bands.File in questo prodotto:
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