Cerato-platanin (CP) and cerato-populin (Pop1) are small non-catalytic proteins produced by the ascomycetes Ceratocystis platani and C. populicola. C. platani is responsible for the canker stain disease of plane trees, and C. populicola for the black canker of poplar trees. CP and Pop1 are PAMPs (pathogen-associated molecular patterns) inducing typical defense responses in various host and non-host plants. CP causes the up-regulation of defence-related genes in Platanus acerifolia leaves treated for 48 hours. However, other defence-related events like cell plasmolysis and localized resistance occur before 48 hours. With the present work, the transcriptional changes caused by CP in P. acerifolia leaves were studied during the first 24 hours. A cDNA microarray containing both P. acerifolia transcripts induced by CP after 48 hours (Baccelli et al. 2008, Fontana et al. 2008) and poplar transcripts induced by cold or ozone (Rizzo et al. 2007, Maestrini et al. 2009) was designed to study the gene regulation induced by CP at 24 hours. Out of 318 cDNA sequences present on the microarray, 131 turned out to be differentially regulated in P. acerifolia leaves after 24 hours of treatment with CP. Out of the 131 modulated transcripts, 86 new sequences of P. acerifolia differentially regulated by CP, 35 up-regulated and 51 down-regulated, were found; these new CP-responsive genes were grouped in seven functional categories. Interestingly, the microarray contained 100 P. acerifolia transcripts known to be up-regulated by CP after 48 hours of treatment, but only 36 were also up-regulated after 24 hours. In order to confirm the microarray analysis, quantitative PCRs (qPCRs) were performed on five selected transcripts. Although with some differences, qPCR data validated the microarray results. The transcripts used to validate the microarray result were also selected to carry out a time-course analysis in P. acerifolia leaves treated with CP also comparing the eliciting activity of CP with Pop1. CP and Pop1 were able to induce early transcriptional changes, confirming that PAMPs act at gene transcription level very quickly after their perception.
Early transcription of defence-related genes in Platanus acerifolia leaves after treatment with cerato-platanin and cerato-populin
BERNARDI, RODOLFO
2012-01-01
Abstract
Cerato-platanin (CP) and cerato-populin (Pop1) are small non-catalytic proteins produced by the ascomycetes Ceratocystis platani and C. populicola. C. platani is responsible for the canker stain disease of plane trees, and C. populicola for the black canker of poplar trees. CP and Pop1 are PAMPs (pathogen-associated molecular patterns) inducing typical defense responses in various host and non-host plants. CP causes the up-regulation of defence-related genes in Platanus acerifolia leaves treated for 48 hours. However, other defence-related events like cell plasmolysis and localized resistance occur before 48 hours. With the present work, the transcriptional changes caused by CP in P. acerifolia leaves were studied during the first 24 hours. A cDNA microarray containing both P. acerifolia transcripts induced by CP after 48 hours (Baccelli et al. 2008, Fontana et al. 2008) and poplar transcripts induced by cold or ozone (Rizzo et al. 2007, Maestrini et al. 2009) was designed to study the gene regulation induced by CP at 24 hours. Out of 318 cDNA sequences present on the microarray, 131 turned out to be differentially regulated in P. acerifolia leaves after 24 hours of treatment with CP. Out of the 131 modulated transcripts, 86 new sequences of P. acerifolia differentially regulated by CP, 35 up-regulated and 51 down-regulated, were found; these new CP-responsive genes were grouped in seven functional categories. Interestingly, the microarray contained 100 P. acerifolia transcripts known to be up-regulated by CP after 48 hours of treatment, but only 36 were also up-regulated after 24 hours. In order to confirm the microarray analysis, quantitative PCRs (qPCRs) were performed on five selected transcripts. Although with some differences, qPCR data validated the microarray results. The transcripts used to validate the microarray result were also selected to carry out a time-course analysis in P. acerifolia leaves treated with CP also comparing the eliciting activity of CP with Pop1. CP and Pop1 were able to induce early transcriptional changes, confirming that PAMPs act at gene transcription level very quickly after their perception.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
