An electroanalytical method was developed for the determination of the phytohormone indole-3-acetic acid in water using a glassy-carbon electrode. The differential pulse voltammetry was combined with a purification step which involved the extraction of plant tissue and the separation of plant pigments by passing through column Oasis MCX. Indole-3-acetic acid can be determined under optimum conditions with a limit of quantification (LOQ) 2.7 106 mol L1. The linearity range was from 1.8106 to 6.6104 mol L1. The electroanalytical determination of indole-3-acetic acid is selective, fast and economic. The presence of other phytohormones as abscisic acid and cytokinins as isopentenyladenine, isopentenyladenosine, trans-zeatin, zeatin riboside does not interfere the determination of indole-3-acetic acid in plant sample.

Determination of Plant Hormone Indole-3-Acetic Acid in Aqueous Solution

GIANNARELLI, STEFANIA;MUSCATELLO, BEATRICE
2013-01-01

Abstract

An electroanalytical method was developed for the determination of the phytohormone indole-3-acetic acid in water using a glassy-carbon electrode. The differential pulse voltammetry was combined with a purification step which involved the extraction of plant tissue and the separation of plant pigments by passing through column Oasis MCX. Indole-3-acetic acid can be determined under optimum conditions with a limit of quantification (LOQ) 2.7 106 mol L1. The linearity range was from 1.8106 to 6.6104 mol L1. The electroanalytical determination of indole-3-acetic acid is selective, fast and economic. The presence of other phytohormones as abscisic acid and cytokinins as isopentenyladenine, isopentenyladenosine, trans-zeatin, zeatin riboside does not interfere the determination of indole-3-acetic acid in plant sample.
2013
Jana, Bulickova; Romana, Sokolova; Giannarelli, Stefania; Muscatello, Beatrice
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/159545
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