A rapid and sensitive HPLC method has been developed for the quantification of mirtazapine (MRZ), a noradrenergic and specific serotonergic inhibitor antidepressant (NaSSA) and its two major metabolites N-desmethylmirtazapine (NDM) and 8-hydroxymirtazapine (8-OHM) in human plasma. The separation was achieved using Chromolith C18 columns and a mobile phase of acetonitrile: phosphate buffer (pH=3, 20:80, v/v) in isocratic mode at a flow rate of 2 ml/min. A fluorescence detector was set at 290 and 350 nm for excitation and emission, respectively. Zolpidem was used as the internal standard. Liquid-liquid extraction was applied for sample clean up. All analytes were eluted in less than 5 minutes with LOQ of 1 ng/ml for MRZ and 2 ng/ml for both NDM and 8-OHM . The developed method was successfully applied to quantify MRZ and its metabolites in plasma of a healthy volunteer.
A RAPID AND SENSITIVE HPLC-FLUORESCENCE METHOD FOR DETERMINATION OF MIRTAZAPINE AND ITS TWO MAJOR METABOLITES IN HUMAN PLASMA
GIORGI, MARIO;
2014-01-01
Abstract
A rapid and sensitive HPLC method has been developed for the quantification of mirtazapine (MRZ), a noradrenergic and specific serotonergic inhibitor antidepressant (NaSSA) and its two major metabolites N-desmethylmirtazapine (NDM) and 8-hydroxymirtazapine (8-OHM) in human plasma. The separation was achieved using Chromolith C18 columns and a mobile phase of acetonitrile: phosphate buffer (pH=3, 20:80, v/v) in isocratic mode at a flow rate of 2 ml/min. A fluorescence detector was set at 290 and 350 nm for excitation and emission, respectively. Zolpidem was used as the internal standard. Liquid-liquid extraction was applied for sample clean up. All analytes were eluted in less than 5 minutes with LOQ of 1 ng/ml for MRZ and 2 ng/ml for both NDM and 8-OHM . The developed method was successfully applied to quantify MRZ and its metabolites in plasma of a healthy volunteer.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
