The feline immunodeficiency virus (FIV) induces syncytia in Crandell feline kidney (CrFK) cells grown in low fetal bovine serum-containing medium. This finding has allowed the development of sensitive FIV titration and neutralization assays using syncytium formation as an indicator of infection. In this report we examine several variables that can influence number and size of syncytia. In addition, by performing assays under rigidly controlled culture conditions, we confirm that serum neutralization assays based on FIV-induced syncytium formation in CrFK cells detect broadly reactive neutralizing antibodies.
EXAMINATION OF VARIABLES AFFECTING SYNCYTIUM FORMATION BY, AND SERUM NEUTRALIZATION OF, FELINE IMMUNODEFICIENCY VIRUS ON CRFK CELLS
BANDECCHI, PATRIZIAWriting – Original Draft Preparation
;PISTELLO, MAUROWriting – Original Draft Preparation
;
1995-01-01
Abstract
The feline immunodeficiency virus (FIV) induces syncytia in Crandell feline kidney (CrFK) cells grown in low fetal bovine serum-containing medium. This finding has allowed the development of sensitive FIV titration and neutralization assays using syncytium formation as an indicator of infection. In this report we examine several variables that can influence number and size of syncytia. In addition, by performing assays under rigidly controlled culture conditions, we confirm that serum neutralization assays based on FIV-induced syncytium formation in CrFK cells detect broadly reactive neutralizing antibodies.File in questo prodotto:
Non ci sono file associati a questo prodotto.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
