Objectives: The presence of tubby protein in human lymphocytes was investigated and their electrophoretic mobility property between normal weight and obese subjects was compared. Design and methods: 2-DE proteome map of lymphocytes has been generated and western blot analysis was conducted using anti-tub polyclonal antibody. Results and conclusions: We found the presence of tubby protein both in normal weight and in obese subjects; however in the latter an isoelectric point shift toward the acidic end was observed. (c) 2007 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
Tubby protein in human lymphocytes from normal weight and obese subjects
GIANNACCINI, GINO;GIUSTI, LAURA;SANTINI, FERRUCCIO;BETTI, LAURA;CIREGIA, FEDERICA;LUCACCHINI, ANTONIO;VITTI, PAOLO;
2007-01-01
Abstract
Objectives: The presence of tubby protein in human lymphocytes was investigated and their electrophoretic mobility property between normal weight and obese subjects was compared. Design and methods: 2-DE proteome map of lymphocytes has been generated and western blot analysis was conducted using anti-tub polyclonal antibody. Results and conclusions: We found the presence of tubby protein both in normal weight and in obese subjects; however in the latter an isoelectric point shift toward the acidic end was observed. (c) 2007 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.File in questo prodotto:
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