4-(Dimethylamino)pyridine-coated gold nanoparticles (DMAP-Au NPs) were synthesized, characterised and their interaction with DNA and living cells was analysed. Concerning the interaction of the DMAP-Au NPs with DNA, absorbance titrations indicate that a non-covalent interaction between DNA and the external surface of the NPs does take place. The binding constant was evaluated to be (2.8 ± 0.8) 9 105 M-1. Exposure of cultured cells to NPs revealed a dose-dependent effect on cell proliferation which was increased or reduced in dependence of DMAP-Au NPs concentrations. Subcellular localisation by transmission electron microscopy showed mitochondrial and nuclear localisations of NPs, thus suggesting their direct involvement in the mitochondrial alterations observed and a possible direct interaction with cell DNA. These findings clearly indicate that DMAP-Au NPs can strongly interact with living cells and confirm the importance of systematic evaluations of NPs properties, also in the perspective of their arising diagnostic and therapeutic applications

Analysis of 4-dimethylaminopyridine (DMAP)-gold nanoparticles behaviour in solution and of their interaction with calf thymus DNA and living cells

BIVER, TARITA;CORTI, ALESSANDRO;LORENZINI, EVELINA;MASINI, MATILDE;PAOLICCHI, ALDO;PUCCI, ANDREA;RUGGERI, GIACOMO;
2012-01-01

Abstract

4-(Dimethylamino)pyridine-coated gold nanoparticles (DMAP-Au NPs) were synthesized, characterised and their interaction with DNA and living cells was analysed. Concerning the interaction of the DMAP-Au NPs with DNA, absorbance titrations indicate that a non-covalent interaction between DNA and the external surface of the NPs does take place. The binding constant was evaluated to be (2.8 ± 0.8) 9 105 M-1. Exposure of cultured cells to NPs revealed a dose-dependent effect on cell proliferation which was increased or reduced in dependence of DMAP-Au NPs concentrations. Subcellular localisation by transmission electron microscopy showed mitochondrial and nuclear localisations of NPs, thus suggesting their direct involvement in the mitochondrial alterations observed and a possible direct interaction with cell DNA. These findings clearly indicate that DMAP-Au NPs can strongly interact with living cells and confirm the importance of systematic evaluations of NPs properties, also in the perspective of their arising diagnostic and therapeutic applications
2012
Biver, Tarita; Corti, Alessandro; N., Eltugral; Lorenzini, Evelina; Masini, Matilde; Paolicchi, Aldo; Pucci, Andrea; Ruggeri, Giacomo; F., Secco; M., Venturini
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/192835
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