The project NUTRASNACK (E.C. F.P.6 contract No FOOD-CT-2005-023044) has the main objective to produce by in vitro cultures secondary metabolites with health benefits, which can be used for fortification of some food products. On the of the key partner is ENERVIT®, a well-known firm in the development of nutrition programs to enhance the performances of athletes and sportsmen. For the purpose of the project a number of plant species have been selected for the study of the possibility of commercial application of their secondary metabolites. This includes basil (Ocimium basilicum), dandelion (Taraxacum officinale), soybean (Glycine max), mint (Mentha piperica) and Leguminosae species (genus Trifolium). For above species the conditions and protocols for the extraction of active principles need to be established. These protocols are aimed at the preparation of extracts for analytical purposes but also for commercial application for separation of large amounts of phytochemicals. For this purpose he extraction with different solvents and the purification of extracts by Reversed Phase C18 or Amberlite XAD4 Solid Phase Extraction. It was shown that in case of green samples two options should be considered. For samples used for analytical purposes the 70% EtOH should be a case of choice. For commercial purposes the water extract is more reasonable. Water extracts contain all active chemicals and allow to avoid chlorophyll presence in the extract. This substantially simplified the method of purification and enrichment. In case of soybean the EtOH extract is necessary as isoflavones are not so readily soluble in water. Further purification can be achieved by SPE.
Extraction and Purification of Active Natural Products from Four Plants Species
PISTELLI, LUISA;BERTOLI, ALESSANDRA;
2007-01-01
Abstract
The project NUTRASNACK (E.C. F.P.6 contract No FOOD-CT-2005-023044) has the main objective to produce by in vitro cultures secondary metabolites with health benefits, which can be used for fortification of some food products. On the of the key partner is ENERVIT®, a well-known firm in the development of nutrition programs to enhance the performances of athletes and sportsmen. For the purpose of the project a number of plant species have been selected for the study of the possibility of commercial application of their secondary metabolites. This includes basil (Ocimium basilicum), dandelion (Taraxacum officinale), soybean (Glycine max), mint (Mentha piperica) and Leguminosae species (genus Trifolium). For above species the conditions and protocols for the extraction of active principles need to be established. These protocols are aimed at the preparation of extracts for analytical purposes but also for commercial application for separation of large amounts of phytochemicals. For this purpose he extraction with different solvents and the purification of extracts by Reversed Phase C18 or Amberlite XAD4 Solid Phase Extraction. It was shown that in case of green samples two options should be considered. For samples used for analytical purposes the 70% EtOH should be a case of choice. For commercial purposes the water extract is more reasonable. Water extracts contain all active chemicals and allow to avoid chlorophyll presence in the extract. This substantially simplified the method of purification and enrichment. In case of soybean the EtOH extract is necessary as isoflavones are not so readily soluble in water. Further purification can be achieved by SPE.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
