The authors investigated the presence of serotonin receptor type 1A (5-HT1A) as labeled by the specific ligand H-3-8 hydroxy-2-(di-N-propylamino)tetralin (H-3-8-OH-DPAT) in saturation experiments, and the expression of the mRNA encoding them, in human peripheral blood mononuclear cells (PBMC). In situ hybridization experiments were performed as well. The results, showing that the binding of [H-3]-8-OH-DPAT to lymphocyte membranes increased linearly up to 100 nM without reaching saturation, may indicate that the H-3-8-OH-DPAT was not specifically labeling the 5-HT1A receptor. By contrast, the expression studies revealed 5-HT1A mRNA in PBMC. These findings suggest that, despite the presence of mRNA, 5-HT1A receptors are not expressed in PBMC, at least in healthy controls.
PRESENCE OF SEROTONIN(1A) (5-HT1A) RECEPTOR MESSENGER-RNA WITHOUT BINDING OF [H-3] 8-OH-DPAT IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS
PALEGO, LIONELLA;PASQUALETTI, MASSIMO;GIANNACCINI, GINO;LUCACCHINI, ANTONIO;NARDI, IRMA;
1995-01-01
Abstract
The authors investigated the presence of serotonin receptor type 1A (5-HT1A) as labeled by the specific ligand H-3-8 hydroxy-2-(di-N-propylamino)tetralin (H-3-8-OH-DPAT) in saturation experiments, and the expression of the mRNA encoding them, in human peripheral blood mononuclear cells (PBMC). In situ hybridization experiments were performed as well. The results, showing that the binding of [H-3]-8-OH-DPAT to lymphocyte membranes increased linearly up to 100 nM without reaching saturation, may indicate that the H-3-8-OH-DPAT was not specifically labeling the 5-HT1A receptor. By contrast, the expression studies revealed 5-HT1A mRNA in PBMC. These findings suggest that, despite the presence of mRNA, 5-HT1A receptors are not expressed in PBMC, at least in healthy controls.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
