GLYCOGEN RICH CARCINOMA OF CANINE MALE AND FEMALE MAMMARY GLAND Muscatello L.V.*[2], Sarli G.[2], Beha G.[2], Benazzi C.[2], Millanta F.[1], Poli A.[1], Asproni P.[1], Giudice C.[3], Levi M.[2], Brunetti B.[2] [1]Dip. Scienze Veterinarie ~ Pisa, [2]DIMEVET ~ Bologna, [3]Dip. Scienze Veterinarie Sanità Pubblica ~ Milano Glycogen rich clear cell carcinoma (GRCC) is a rare subtype of human invasive mammary gland (MG) carcinoma, in which at least 90% of the neoplastic cells have clear cytoplasm containing glycogen(1). The aim of this study was to describe the histological, histochemical and immunohistochemical (IHC) features of GRCC of canine MG. Serial formalin fixed paraffin embedded tissue sections of two GRCC canine mammary carcinoma, one female (case I) and one male (case II) were stained with H&E, PAS, PAS diastase (dPAS), and Alcian Blue (AB). IHC was performed with anti-ER, -PR, -cerbB2, -CK19, -CK14, -CK5/6, -p63, -vimentin, -SMA, -calponin, -S100, -EGFR, -c-KIT, -E-cad and -ki67 antibodies. Sudan III was carried out on formalin-fixed frozen tissue in case II. Histologically, 90% of neoplastic cells showed sharply distinct borders, clear or finely granular cytoplasm and low N:C ratio. In case II, residual 10% of the neoplastic cells had lipid-like vacuolated cytoplasm. In both tumors, the cytoplasm resulted strongly positive with PAS. Treatment with diastase abolished PAS reactivity. Case I showed PAS+ and dPAS- staining also in lymph node metastasis. In case II, 10% of neoplastic cells were positive to Sudan III. No stain with AB was obtained. Case I showed positivity for CK19 and CK5/6, negativity for ER, PR and c-erbB2, resulting a basal-like phenotype in primary tumor and lymph node metastasis. They were both positive for EGFR, E-cad, c-KIT, and weakly for calponin. Case II was a basal-like phenotype, presenting CK 19, E-cad, c-KIT, weak CK14 and strong vimentin positivity. The proliferative ki67 index was 26.75% in case I and 8.2% in case II. Based on the morphology, typical features of human MG GRCC are the “fried eggs appearance”, clear cytoplasm and small dark punctate nuclei (2).On the best of our knowledge, this is the first report regarding GRCC in canine MG. The diagnosis was confirmed by PAS+ of intracytoplasmic glycogen granules and lack of stain with dPAS. Case II was considered a GRCC with lipid rich differentiation. Both cases had a basal-like phenotype and the expression of EGFR and c-KIT was suggestive of an association of cell proliferation with signal transduction of surface molecules (3). GRCC can be considered a new rare histological subtype of canine mammary tumors, with clear cytoplasm, PAS+ and dPAS-, expressing the triple negative phenotype, a tumor with clinical aggressive behavior that should be differentiated from lipid rich carcinoma. 1. Tavassoli, Deville. Lyon: IARC Press, 2003 2. Rosen, Philadelphia. LWW press, 2001 3. Kim et al., Yonsei Med J 53:1142-1146, 2012 ANATOMIA PATOLOGICA Canine, Mammary carcinoma, Glycogen rich

GLYCOGEN RICH CARCINOMA OF CANINE MALE AND FEMALE MAMMARY GLAND

MILLANTA, FRANCESCA;POLI, ALESSANDRO;
2014-01-01

Abstract

GLYCOGEN RICH CARCINOMA OF CANINE MALE AND FEMALE MAMMARY GLAND Muscatello L.V.*[2], Sarli G.[2], Beha G.[2], Benazzi C.[2], Millanta F.[1], Poli A.[1], Asproni P.[1], Giudice C.[3], Levi M.[2], Brunetti B.[2] [1]Dip. Scienze Veterinarie ~ Pisa, [2]DIMEVET ~ Bologna, [3]Dip. Scienze Veterinarie Sanità Pubblica ~ Milano Glycogen rich clear cell carcinoma (GRCC) is a rare subtype of human invasive mammary gland (MG) carcinoma, in which at least 90% of the neoplastic cells have clear cytoplasm containing glycogen(1). The aim of this study was to describe the histological, histochemical and immunohistochemical (IHC) features of GRCC of canine MG. Serial formalin fixed paraffin embedded tissue sections of two GRCC canine mammary carcinoma, one female (case I) and one male (case II) were stained with H&E, PAS, PAS diastase (dPAS), and Alcian Blue (AB). IHC was performed with anti-ER, -PR, -cerbB2, -CK19, -CK14, -CK5/6, -p63, -vimentin, -SMA, -calponin, -S100, -EGFR, -c-KIT, -E-cad and -ki67 antibodies. Sudan III was carried out on formalin-fixed frozen tissue in case II. Histologically, 90% of neoplastic cells showed sharply distinct borders, clear or finely granular cytoplasm and low N:C ratio. In case II, residual 10% of the neoplastic cells had lipid-like vacuolated cytoplasm. In both tumors, the cytoplasm resulted strongly positive with PAS. Treatment with diastase abolished PAS reactivity. Case I showed PAS+ and dPAS- staining also in lymph node metastasis. In case II, 10% of neoplastic cells were positive to Sudan III. No stain with AB was obtained. Case I showed positivity for CK19 and CK5/6, negativity for ER, PR and c-erbB2, resulting a basal-like phenotype in primary tumor and lymph node metastasis. They were both positive for EGFR, E-cad, c-KIT, and weakly for calponin. Case II was a basal-like phenotype, presenting CK 19, E-cad, c-KIT, weak CK14 and strong vimentin positivity. The proliferative ki67 index was 26.75% in case I and 8.2% in case II. Based on the morphology, typical features of human MG GRCC are the “fried eggs appearance”, clear cytoplasm and small dark punctate nuclei (2).On the best of our knowledge, this is the first report regarding GRCC in canine MG. The diagnosis was confirmed by PAS+ of intracytoplasmic glycogen granules and lack of stain with dPAS. Case II was considered a GRCC with lipid rich differentiation. Both cases had a basal-like phenotype and the expression of EGFR and c-KIT was suggestive of an association of cell proliferation with signal transduction of surface molecules (3). GRCC can be considered a new rare histological subtype of canine mammary tumors, with clear cytoplasm, PAS+ and dPAS-, expressing the triple negative phenotype, a tumor with clinical aggressive behavior that should be differentiated from lipid rich carcinoma. 1. Tavassoli, Deville. Lyon: IARC Press, 2003 2. Rosen, Philadelphia. LWW press, 2001 3. Kim et al., Yonsei Med J 53:1142-1146, 2012 ANATOMIA PATOLOGICA Canine, Mammary carcinoma, Glycogen rich
2014
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/466072
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