Intramuscular fat (IMF) is one of the most important traits influencing meat quality as its level affects juiciness, tastiness and, in general, consumer acceptance. IMF content can change with diet and is also under genetic control. Several studies reported in PigQTLdb showed the presence of significant QTL associated to intramuscular fat content but relevant causative mutations haven’t been so far detected. With the aim to detect association between regions of porcine genome and fat content we performed a genome scan with the Illumina PorcineSNP60 BeadChip using 912 Italian Large White pigs included in the Sib Test genetic evaluation program of the Italian Association of Pig Breeders (ANAS). These pigs were reared on the same herd, feed on the same diet, and slaughtered on the same abattoir during year 2012. Intramuscular fat content was measured on Semimembranosus muscle using Ankom method based on Soxhlet technique. The average fat content was 1.98±1.09% and minimum and maximum values were 0.51% and 8.64%, respectively. The genotypes for 61,565 SNPs were obtained and these data were filtered using PLINK toolset to remove animals without genetic evaluation and MIND>0.1. Additional criteria were used to filter the SNP data according to MAF<0.01, GENO>0.1, HWE <0.001. The final dataset was composed by 890 pigs and 49,677 SNPs and population stratification was examined using IBS approach. Preliminary results showed that some of the most significant putative markers for IMF content mapped on chromosome 1. Further analyses are in progress in order to complete and validate the obtained results. The Authors wish to thank ANAS for providing samples and data. This research was supported by project AGER-HEPIGET (grant N. 2011-0279).

Genome wide association study for intramuscular fat in Italian Large White pigs using SNP60K chip

SERRA, ANDREA;
2014-01-01

Abstract

Intramuscular fat (IMF) is one of the most important traits influencing meat quality as its level affects juiciness, tastiness and, in general, consumer acceptance. IMF content can change with diet and is also under genetic control. Several studies reported in PigQTLdb showed the presence of significant QTL associated to intramuscular fat content but relevant causative mutations haven’t been so far detected. With the aim to detect association between regions of porcine genome and fat content we performed a genome scan with the Illumina PorcineSNP60 BeadChip using 912 Italian Large White pigs included in the Sib Test genetic evaluation program of the Italian Association of Pig Breeders (ANAS). These pigs were reared on the same herd, feed on the same diet, and slaughtered on the same abattoir during year 2012. Intramuscular fat content was measured on Semimembranosus muscle using Ankom method based on Soxhlet technique. The average fat content was 1.98±1.09% and minimum and maximum values were 0.51% and 8.64%, respectively. The genotypes for 61,565 SNPs were obtained and these data were filtered using PLINK toolset to remove animals without genetic evaluation and MIND>0.1. Additional criteria were used to filter the SNP data according to MAF<0.01, GENO>0.1, HWE <0.001. The final dataset was composed by 890 pigs and 49,677 SNPs and population stratification was examined using IBS approach. Preliminary results showed that some of the most significant putative markers for IMF content mapped on chromosome 1. Further analyses are in progress in order to complete and validate the obtained results. The Authors wish to thank ANAS for providing samples and data. This research was supported by project AGER-HEPIGET (grant N. 2011-0279).
2014
9789086862481
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/657289
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