(2S,3S )-2-Amino-3-hydroxy-5-(4-nitrophenoxy )pentanoic acid ( 5 ) was prepared stereoselectively as the N- Fmoc, O-(tert-butyl )-protected derivative 5a in eleven steps from ethyl ( E)-4-benzyloxypent-2-enoate (6 ) . This protected amino acid was used for the solid-phase peptide synthesis of oligopeptides, which serve as sequence- specific chromogenic protease substrates when used in the presence of NaIO4 and bovine serum albumin. The peptide 1 ( KRAVNle 5EANleNH2 ( Nle norleucine)) allows detection of HIV-protease activity spectro-photometrically at 405 nm.

A Selective HIV-Protease Assay Based on a Chromogenic Aminoacid

CROTTI, PAOLO;
2002-01-01

Abstract

(2S,3S )-2-Amino-3-hydroxy-5-(4-nitrophenoxy )pentanoic acid ( 5 ) was prepared stereoselectively as the N- Fmoc, O-(tert-butyl )-protected derivative 5a in eleven steps from ethyl ( E)-4-benzyloxypent-2-enoate (6 ) . This protected amino acid was used for the solid-phase peptide synthesis of oligopeptides, which serve as sequence- specific chromogenic protease substrates when used in the presence of NaIO4 and bovine serum albumin. The peptide 1 ( KRAVNle 5EANleNH2 ( Nle norleucine)) allows detection of HIV-protease activity spectro-photometrically at 405 nm.
2002
F., Badalassi; Crotti, Paolo; H. K., Nguyen; J. L., Reymond
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/70417
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? 16
social impact