Application of a novel ionization technique, APPI, to the analysis of complex matrices. An APPI source has been built in collaboration with the mass spectrometry facility of the University of Groningen (NL), coordinated by Dr. Andries P. Bruins. Such a source has been adapted to be used on a PE Sciex API III plus triple quadrupole mass spectrometer. The main target of this research was the evaluation of the performance of the source, the study of the influence of some mechanical solutions, and an investigation on the fields of applications, also in term of comparison with Ionspray (ISI) and Atmospheric Pressure Chemical Ionization (APCI). Most of the experiments were performed by Flow Injection Analysis (FIA), taking advantage of tandem mass spectrometry in Selected Reaction Monitoring (SRM) mode. We used fairly standard conditions for ISI and APCI, both in positive and negative ion mode, depending on the analyte examined. The APPI conditions were tuned starting from the APCI conditions. Toluene was used as a dopant for APPI experiments, flowing at 30 .micro.l/min, with a mobile phase flow rate of 200 .micro.l/min. We started the comparison of the different ionization techniques taking into consideration some classes of food components that, in principle, could be considered amenable to APPI. In particular we tested the methods on some phenolic antioxidants present in wine (catechin, quercetin and resveratrol), on one present in olive oil (oleuropein), on .alpha.-, .gamma.- and .delta.-tocopherols, on some components present in essential oils from citrus fruits (coumarins, psoralenes and polymethoxyflavons). Furthermore, we studied the application to some different classes of compounds, and among them polychlorobiphenyls in some matrices.
Evaluation of a Custom Built Atmospheric Pressure PhotoIonization (APPI) Source
SABA, ALESSANDRO;
2002-01-01
Abstract
Application of a novel ionization technique, APPI, to the analysis of complex matrices. An APPI source has been built in collaboration with the mass spectrometry facility of the University of Groningen (NL), coordinated by Dr. Andries P. Bruins. Such a source has been adapted to be used on a PE Sciex API III plus triple quadrupole mass spectrometer. The main target of this research was the evaluation of the performance of the source, the study of the influence of some mechanical solutions, and an investigation on the fields of applications, also in term of comparison with Ionspray (ISI) and Atmospheric Pressure Chemical Ionization (APCI). Most of the experiments were performed by Flow Injection Analysis (FIA), taking advantage of tandem mass spectrometry in Selected Reaction Monitoring (SRM) mode. We used fairly standard conditions for ISI and APCI, both in positive and negative ion mode, depending on the analyte examined. The APPI conditions were tuned starting from the APCI conditions. Toluene was used as a dopant for APPI experiments, flowing at 30 .micro.l/min, with a mobile phase flow rate of 200 .micro.l/min. We started the comparison of the different ionization techniques taking into consideration some classes of food components that, in principle, could be considered amenable to APPI. In particular we tested the methods on some phenolic antioxidants present in wine (catechin, quercetin and resveratrol), on one present in olive oil (oleuropein), on .alpha.-, .gamma.- and .delta.-tocopherols, on some components present in essential oils from citrus fruits (coumarins, psoralenes and polymethoxyflavons). Furthermore, we studied the application to some different classes of compounds, and among them polychlorobiphenyls in some matrices.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
