Phenylpropanoid consumption is well-known to be associated with a reduced risk of several human diseases. Many studies demonstrated that UV-B may influence phenylpropanoid metabolism. This work investigates how UV-B radiation can modulate the transcription of different genes involved in the phenylpropanoid pathway, in fruits of the tomato line SA206-1- 2-2 (with the anthocyanin fruit Aft gene), characterised by purple spots on fruit surface, and in its wild type, cv Roma. Fruits, harvested at mature green stage, were irradiated with UV-B (1h a day, 6.08 kJ m-2 d-1) until red ripe stage. UV-B treatment induced an overall stimulation of the flavonoid biosynthetic pathway in Roma peel. In SA206 peel, early biosynthetic genes (CHS, F3H and F3’H) were down- regulated, leading to a decrease in downstream metabolites. However, F3’5’H and DFR transcription, as well as delphinidin, petunidin and malvidin levels, increased, indicating a metabolic shift towards anthocyanin synthesis in response to UV-B irradiation.

A biochemical and molecular dissection of fruit anthocyanins in anthocyanin-rich tomato mutant after post-harvest UV-B treatment

SANTIN, MARCO;CATOLA, STEFANO;CASTAGNA, ANTONELLA;RANIERI, ANNAMARIA
2016-01-01

Abstract

Phenylpropanoid consumption is well-known to be associated with a reduced risk of several human diseases. Many studies demonstrated that UV-B may influence phenylpropanoid metabolism. This work investigates how UV-B radiation can modulate the transcription of different genes involved in the phenylpropanoid pathway, in fruits of the tomato line SA206-1- 2-2 (with the anthocyanin fruit Aft gene), characterised by purple spots on fruit surface, and in its wild type, cv Roma. Fruits, harvested at mature green stage, were irradiated with UV-B (1h a day, 6.08 kJ m-2 d-1) until red ripe stage. UV-B treatment induced an overall stimulation of the flavonoid biosynthetic pathway in Roma peel. In SA206 peel, early biosynthetic genes (CHS, F3H and F3’H) were down- regulated, leading to a decrease in downstream metabolites. However, F3’5’H and DFR transcription, as well as delphinidin, petunidin and malvidin levels, increased, indicating a metabolic shift towards anthocyanin synthesis in response to UV-B irradiation.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/812798
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