La vite nel Medioevo in Maremma: caratterizzazione morfologica e genotipizzazione di vinaccioli del quindicesimo secolo attraverso marcatori molecolari a DNA

In a recent excavation carried out under the Cathedral of Grosseto, was found a "well throw" dating back to the fifteenth century, containing several archaeological finds and plant material belonging to various botanical species, mainly tree fruit species, including the grapevine. There were recovered more than 200 seeds, which were individually cataloged and photographed to be subjected to ampelometrical analysis. Some of these seeds have been subjected to the analysis of SSR profiles. The DNA was extracted from each seed, quantified by real-time PCR and used to amplify the 14 SSR loci including the 9 selected at international level. The results show considerable variability in both the quantity and quality of DNA between different seeds, and it was possible to obtain reliable SSR profiles and postulate some relationships with the varieties currently grown in Tuscany.