Difficulties have been reported in quantitating 3-iodothyronamine (T1AM) in blood or serum, and tentatively attributed to problems in extraction or other pre-analytical steps. For this reason even cell culture experiments have often be performed with unphysiological protein-free media. The aim of this study was to evaluate the recovery of exogenous T1AM added to a standard cell culture medium, namely DMEM supplemented with fetal bovine serum (FBS), and to other biological matrices.
Recovery of 3-iodothyronamine and derivatives in biological matrices: problems and pitfalls
GHELARDONI, SANDRASecondo
;SABA, ALESSANDRO;CHIELLINI, GRAZIAPenultimo
;ZUCCHI, RICCARDO
Ultimo
2017-01-01
Abstract
Difficulties have been reported in quantitating 3-iodothyronamine (T1AM) in blood or serum, and tentatively attributed to problems in extraction or other pre-analytical steps. For this reason even cell culture experiments have often be performed with unphysiological protein-free media. The aim of this study was to evaluate the recovery of exogenous T1AM added to a standard cell culture medium, namely DMEM supplemented with fetal bovine serum (FBS), and to other biological matrices.File in questo prodotto:
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