Desmosomes are epithelial adhesion structures that assure the mechanical cell-cell adhesion, by linking the intermediate filaments of two adjacent cells. Several proteins have been characterised of components of these structures and, among them, desmogleins are known to be the autoantigens in some autoimmune deseases that go by the name of phemphigus complex deseases. In particular Desmoglein 1 is the target antigen in Pemphigus foliaceus. This study aims at revealing the presence of Desmoiglein 1 in the ovine muzzle skin using a commercial monoclonal antibody, raised against human desmoglein-1, on samples obtained from sheep muzzle. Immunohistochemical analyses (streptavidin-peroxidase method) and immunoblotting analyses (chemiluminescence method ) have been performed. Immunohoistochemical evaluation allowed for detection of a pericellular staining of cheratinocyte cell membranes in the spinous and basal layers of epidermis. A positive immunoreaction was obtained when the anti-Dsg-1 antibody was reacted with human cheek epidermis used as positive control. Specificity of the immune reaction was confirmed by the lack of staining in samples incubated with the anti-vimentin antibody used as negative control. Densitometric analyses of the chemiluminescence film showed three bands. One of them could be referred to Desmoglein 1, the second one to Desmoglein 3 and the last one to a Desmoglein-Plakoglobin complex

Desmoglein 1 in ovine muzzle skin detected by immunohistochemistry and immunoblotting

MIRAGLIOTTA, VINCENZO;COLI, ALESSANDRA;STORNELLI, MARIA RITA;GIANNESSI, ELISABETTA;RICCIARDI, MARIA PAOLA
2005-01-01

Abstract

Desmosomes are epithelial adhesion structures that assure the mechanical cell-cell adhesion, by linking the intermediate filaments of two adjacent cells. Several proteins have been characterised of components of these structures and, among them, desmogleins are known to be the autoantigens in some autoimmune deseases that go by the name of phemphigus complex deseases. In particular Desmoglein 1 is the target antigen in Pemphigus foliaceus. This study aims at revealing the presence of Desmoiglein 1 in the ovine muzzle skin using a commercial monoclonal antibody, raised against human desmoglein-1, on samples obtained from sheep muzzle. Immunohistochemical analyses (streptavidin-peroxidase method) and immunoblotting analyses (chemiluminescence method ) have been performed. Immunohoistochemical evaluation allowed for detection of a pericellular staining of cheratinocyte cell membranes in the spinous and basal layers of epidermis. A positive immunoreaction was obtained when the anti-Dsg-1 antibody was reacted with human cheek epidermis used as positive control. Specificity of the immune reaction was confirmed by the lack of staining in samples incubated with the anti-vimentin antibody used as negative control. Densitometric analyses of the chemiluminescence film showed three bands. One of them could be referred to Desmoglein 1, the second one to Desmoglein 3 and the last one to a Desmoglein-Plakoglobin complex
2005
Miragliotta, Vincenzo; Donadio, E; Coli, Alessandra; Stornelli, MARIA RITA; Giannessi, Elisabetta; Ricciardi, MARIA PAOLA
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/98300
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