Cerato-platanin (CP) is produced from the Ascomycete Ceratocystis fimbriata (Ell. and Halst.) Davidson f. sp. platani Walter (Cfp) that causes the canker stain of the plane trees (Boddi et al. 2004, Pazzagli et al. 1999 and 2006a). In in vitro experimental conditions CP self-assembles and interacts with the host plane leaves by eliciting phytoalexin synthesis, cell plasmolysis and necrosis and restriction of Cfp growth (Bennici et al. 2005, Pazzagli et al 2006b, Santini et al 2006, Scala et al. 2004). We have applied the suppression-subtractive hybridisation (SSH) methodology for isolation and characterisation of genes induced after CP treatment for 48 hours. The data show an intense metabolic activity and many genes differentially expressed, the most part of which code for: 1) defence and/or stress related proteins; 2) proteins involved in the protein synthesis/turnover; 3) proteins belonging to cell primary metabolism and 4) proteins involved in the signalling pathway s. We have analysed some of them by relative PCR using total RNA from treated leaves with CP and Cfp conidia at different times. From the results the CP has been shown to stimulate defence responses.
Evaluating the expression profile in plane leaves treated with cerato-platanin and C. fimbriata f. sp. platani conidia
BERNARDI, RODOLFO;
2006-01-01
Abstract
Cerato-platanin (CP) is produced from the Ascomycete Ceratocystis fimbriata (Ell. and Halst.) Davidson f. sp. platani Walter (Cfp) that causes the canker stain of the plane trees (Boddi et al. 2004, Pazzagli et al. 1999 and 2006a). In in vitro experimental conditions CP self-assembles and interacts with the host plane leaves by eliciting phytoalexin synthesis, cell plasmolysis and necrosis and restriction of Cfp growth (Bennici et al. 2005, Pazzagli et al 2006b, Santini et al 2006, Scala et al. 2004). We have applied the suppression-subtractive hybridisation (SSH) methodology for isolation and characterisation of genes induced after CP treatment for 48 hours. The data show an intense metabolic activity and many genes differentially expressed, the most part of which code for: 1) defence and/or stress related proteins; 2) proteins involved in the protein synthesis/turnover; 3) proteins belonging to cell primary metabolism and 4) proteins involved in the signalling pathway s. We have analysed some of them by relative PCR using total RNA from treated leaves with CP and Cfp conidia at different times. From the results the CP has been shown to stimulate defence responses.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
