Purpose. To investigate the effects of mydriasis obtained with topical 0.5% tropicamide (Visumidriatic 0.5%, Visufarma s.p.a., Rome, Italy) on retinal vascular parameters evaluated by the retinal imaging software VAMPIRE® in cats. Methods. A longitudinal study on 40 clinically normal adult cats of both sexes was performed. Topical 0.5% tropicamide was instilled to dilate the right pupil only. The left eye was used as a control. Before pharmacological dilation (T0), infrared pupillometry of both pupil was performed and fundus images were taken from both eyes. Right eye fundus images were then captured 30 minutes after topical instillation of tropicamide (T1), when mydriasis was achieved. The vessel diameters (4 veins and 4 arteries) were measured with Vampire® annotation tool adapted for measuring the feline fundus. After normality assessment, t-test was use to analyze mean difference for vascular parameters of left and right eye; paired sample t-test was used to test the mean difference for the same vascular parameters at T0 and T1. Statistical significance level was set at alpha=0.05 with Bonferroni correction accounting for multiple comparisons. Results. Right and left eye showed no statistical differences for pupil and vascular parameters measurements at T0. At T1, only one artery measurement showed a significant difference with a mild mean vasoconstriction of about 4% (6.28±0.84 vs 6.04±0.59); none of the other vascular parameters resulted significantly different at this timepoint. Conclusions: Topical 0.5% tropicamide seems not to affect the results of retinal vascular analysis using the retinal imaging software VAMPIRE®.
EFFECTS OF PUPIL DILATION WITH TOPICAL 0.5% TROPICAMIDE ON RETINAL VASCULAR PARAMETERS ASSESSED BY VAMPIRE® (VASCULAR ASSAY AND MEASUREMENT PLATFORM FOR IMAGES OF THE RETINA) SOFTWARE IN HEALTHY CATS
Barsotti G.
2020-01-01
Abstract
Purpose. To investigate the effects of mydriasis obtained with topical 0.5% tropicamide (Visumidriatic 0.5%, Visufarma s.p.a., Rome, Italy) on retinal vascular parameters evaluated by the retinal imaging software VAMPIRE® in cats. Methods. A longitudinal study on 40 clinically normal adult cats of both sexes was performed. Topical 0.5% tropicamide was instilled to dilate the right pupil only. The left eye was used as a control. Before pharmacological dilation (T0), infrared pupillometry of both pupil was performed and fundus images were taken from both eyes. Right eye fundus images were then captured 30 minutes after topical instillation of tropicamide (T1), when mydriasis was achieved. The vessel diameters (4 veins and 4 arteries) were measured with Vampire® annotation tool adapted for measuring the feline fundus. After normality assessment, t-test was use to analyze mean difference for vascular parameters of left and right eye; paired sample t-test was used to test the mean difference for the same vascular parameters at T0 and T1. Statistical significance level was set at alpha=0.05 with Bonferroni correction accounting for multiple comparisons. Results. Right and left eye showed no statistical differences for pupil and vascular parameters measurements at T0. At T1, only one artery measurement showed a significant difference with a mild mean vasoconstriction of about 4% (6.28±0.84 vs 6.04±0.59); none of the other vascular parameters resulted significantly different at this timepoint. Conclusions: Topical 0.5% tropicamide seems not to affect the results of retinal vascular analysis using the retinal imaging software VAMPIRE®.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
