Cerato-platanin (CP), a 120 amino acid protein produced by the ascomycete Ceratocystis platani (Cep), the causal agent of plane canker stain, is the founder member of the recently identified cerato-platanin protein family pfam07249. CP is located in the cell walls of Cep mycelium, is secreted early in culture, contains four cysteines (S-S bridged), is moderately hydrophobic and is able to self-aggregate according to a hierarchical aggregation model. The DNA sequence of the CP gene contains an intron of 59 bp. CP elicits phytoalexin synthesis and/or cell necrosis in host and in non-host plant tissues. The new data presented in the present work include: (i) plane leaves pre-treated with 510-5 M CP restrict Cep growth and overexpress numerous defense-related genes, such as Rar1 protein, b-1,3 glucanase, thaumatin, lipid transfer protein, etc.; (ii) the sequences of the CP-homologs encoded by C. populicola, C. cacaofunesta and C. fimbriata (cloned from Coffea arabica, Mangifera indica, Crotolaria juncea, Fagus sp.) were obtained on the basis of the sequences of the hortologs of the CP gene; (iii) the CP-homolog from C. populicola, named “cerato-populin” was purified and characterized with regard to both structure and resistance-inducing activity; (iv) the 3D structure of CP was determined by multidimensional NMR (Oliveira et al., 2006; Spisni et al., 2007, unpublished information).
New findings on the structure and functions of cerato-platanin and its structural homologs
BERNARDI, RODOLFO;
2007-01-01
Abstract
Cerato-platanin (CP), a 120 amino acid protein produced by the ascomycete Ceratocystis platani (Cep), the causal agent of plane canker stain, is the founder member of the recently identified cerato-platanin protein family pfam07249. CP is located in the cell walls of Cep mycelium, is secreted early in culture, contains four cysteines (S-S bridged), is moderately hydrophobic and is able to self-aggregate according to a hierarchical aggregation model. The DNA sequence of the CP gene contains an intron of 59 bp. CP elicits phytoalexin synthesis and/or cell necrosis in host and in non-host plant tissues. The new data presented in the present work include: (i) plane leaves pre-treated with 510-5 M CP restrict Cep growth and overexpress numerous defense-related genes, such as Rar1 protein, b-1,3 glucanase, thaumatin, lipid transfer protein, etc.; (ii) the sequences of the CP-homologs encoded by C. populicola, C. cacaofunesta and C. fimbriata (cloned from Coffea arabica, Mangifera indica, Crotolaria juncea, Fagus sp.) were obtained on the basis of the sequences of the hortologs of the CP gene; (iii) the CP-homolog from C. populicola, named “cerato-populin” was purified and characterized with regard to both structure and resistance-inducing activity; (iv) the 3D structure of CP was determined by multidimensional NMR (Oliveira et al., 2006; Spisni et al., 2007, unpublished information).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
