Biomasses have received increasing attention because of their availability and because they are a source of energy and chemicals.[1] The three main components of the lignocellulosic cell wall are cellulose, lignin, and hemicellulose. Hydrolysis of lignocellulosic biomass is useful for various applications, such as the synthesis of intermediates for the chemical and pharmaceutical industries, food additives, surfactants, solvents, and polymers. The most used strategy for obtaining cellulose nanocrystals (CNCs) is acid hydrolysis at moderate temperatures in the presence of strong acids, such as H2SO4 or HCl, which can degrade hemicellulose and the amorphous parts of cellulose, allowing crystalline parts to be isolated.[2] This strategy allowed to isolate CNCs from Cynara cardunculus (Figure 1), after mild organosolv delignification. However, acid hydrolysis may be a source of environmental concern, taking into account the large volumesof acid solution used to prepare the nanocrystals. A greener preparation procedure is based on enzymatic hydrolysis, which has a low environmental impact if compared with the previous method.[3] A commercially available enzyme such as cellulase from Aspergillus niger was used in this strategy. Here we show the hydrolysis results of cellulase attack on microcrystalline cellulose (Avicel PH10.1). Future developments of this work will entail the use of the enzymatic degradation strategy to enable cellulose nanocrystal synthesis from delignified biomass. References: [1] T. Giannoni, M. Gelosia, A. Bertini, G. Fabbrizi, A. Nicolini, V. Coccia, P. Iodice, G. Cavalaglio, Sustainability, 13, 8714, (2021). [2] A. Operamolla, C. Mazzuca, L. Capodieci, F. Di Benedetto, L. Severini, M. Titubante, A. Martinelli, V. Castelvetro, L. Micheli, ACS Applied Materials & Interfaces ,13, 44972-44982, (2021). [3] A. Akhtar, A. Anchal, D.Goyal, A. Goyal, Cellulose Chem. Technol.,50, 983-995, (2016).
Laura Spagnuolo
Primo
;Rosarita D'ORsi;Nicola Di Fidio;Claudia Antonetti;Anna Maria Raspolli Galletti;Alessandra Operamolla
Abstract
Biomasses have received increasing attention because of their availability and because they are a source of energy and chemicals.[1] The three main components of the lignocellulosic cell wall are cellulose, lignin, and hemicellulose. Hydrolysis of lignocellulosic biomass is useful for various applications, such as the synthesis of intermediates for the chemical and pharmaceutical industries, food additives, surfactants, solvents, and polymers. The most used strategy for obtaining cellulose nanocrystals (CNCs) is acid hydrolysis at moderate temperatures in the presence of strong acids, such as H2SO4 or HCl, which can degrade hemicellulose and the amorphous parts of cellulose, allowing crystalline parts to be isolated.[2] This strategy allowed to isolate CNCs from Cynara cardunculus (Figure 1), after mild organosolv delignification. However, acid hydrolysis may be a source of environmental concern, taking into account the large volumesof acid solution used to prepare the nanocrystals. A greener preparation procedure is based on enzymatic hydrolysis, which has a low environmental impact if compared with the previous method.[3] A commercially available enzyme such as cellulase from Aspergillus niger was used in this strategy. Here we show the hydrolysis results of cellulase attack on microcrystalline cellulose (Avicel PH10.1). Future developments of this work will entail the use of the enzymatic degradation strategy to enable cellulose nanocrystal synthesis from delignified biomass. References: [1] T. Giannoni, M. Gelosia, A. Bertini, G. Fabbrizi, A. Nicolini, V. Coccia, P. Iodice, G. Cavalaglio, Sustainability, 13, 8714, (2021). [2] A. Operamolla, C. Mazzuca, L. Capodieci, F. Di Benedetto, L. Severini, M. Titubante, A. Martinelli, V. Castelvetro, L. Micheli, ACS Applied Materials & Interfaces ,13, 44972-44982, (2021). [3] A. Akhtar, A. Anchal, D.Goyal, A. Goyal, Cellulose Chem. Technol.,50, 983-995, (2016).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
