The osteochondral junction is a highly organized structure where the articular cartilage and the subchondral bone are intimately connected, which makes it challenging to be replicated in vitro. In this work, we developed and characterized an in vitro osteochondral construct combining a bone and a cartilage scaffold in a dual chamber bioreactor. First, a bone-mimicking gelatin-nanohydroxyapatite scaffold was 3D bioprinted by bioplotting. Then, the cartilage phase was obtained by incorporating human mesenchymal stem cells (hMSCs) in a photocrosslinkable hydrogel of methacrylated gelatin and hyaluronic acid. Live/dead and histological assays showed promising results on hMSCs vitality and differentiation into osseous and chondral linages after four weeks of culture in a dual chamber bioreactor.
Biofabrication and characterization of a biphasic construct to study osteochondral tissue in vitro
Chiesa I.;De Maria C.;Lapomarda A.;Fortunato G. M.;Montemurro F.;Vozzi G.;
2020-01-01
Abstract
The osteochondral junction is a highly organized structure where the articular cartilage and the subchondral bone are intimately connected, which makes it challenging to be replicated in vitro. In this work, we developed and characterized an in vitro osteochondral construct combining a bone and a cartilage scaffold in a dual chamber bioreactor. First, a bone-mimicking gelatin-nanohydroxyapatite scaffold was 3D bioprinted by bioplotting. Then, the cartilage phase was obtained by incorporating human mesenchymal stem cells (hMSCs) in a photocrosslinkable hydrogel of methacrylated gelatin and hyaluronic acid. Live/dead and histological assays showed promising results on hMSCs vitality and differentiation into osseous and chondral linages after four weeks of culture in a dual chamber bioreactor.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
