The co-culture of the human gut microbiota and eukaryotic cells is still a great challenge in biomedical research. Direct exposure to microbes could in fact cause cell infections or death, as microbial metabolites may have cytotoxic effects. Moreover, cells and microbes usually require different culture conditions. Accordingly, this work presents the design, development and performance of a novel micro-dialyser to allow the microfluidic co-culture of eukaryotic cells and microbes thereby assessing their crosstalk while preventing their direct contact.
Design and fabrication of a micro-dialyser for the study of the gut microbiota-tissues cross-talk
De Acutis A.;Daddi C.;Calvigioni M.;Ghelardi E.;De Maria C.;Vozzi G.
2023-01-01
Abstract
The co-culture of the human gut microbiota and eukaryotic cells is still a great challenge in biomedical research. Direct exposure to microbes could in fact cause cell infections or death, as microbial metabolites may have cytotoxic effects. Moreover, cells and microbes usually require different culture conditions. Accordingly, this work presents the design, development and performance of a novel micro-dialyser to allow the microfluidic co-culture of eukaryotic cells and microbes thereby assessing their crosstalk while preventing their direct contact.File in questo prodotto:
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