The aim of this study was the isolation and characterization the microbiota associated with the arbuscular mycorrhizal fungi (AMF) colonizing the roots of Ammophila arenaria. Rhizosphere soil from three A. arenaria plants was collected from a stable dune system in the Migliarino-San Rossore-Massaciuccoli Natural Park, on Tuscan coast. The dominant AMF species retrieved were Racocetra persica, Racocetra fulgida, Acaulospora scrobiculata, Rhizoglomus irregulare and Glomus spp. In particular, the genus Racocetra was the most abundant (68%) in A. arenaria rhizosphere. Illumina MiSeq sequencing of bacteria associated with R. fulgida spores allowed the detection of 9 phyla, mostly represented by Bacillota (89.9%), Actinomycetota (5.5%) and Pseudomonadota (3.4%). Interestingly, most sequences were identified as Mollicutes/mycoplasma-related endobacteria. A richer bacterial community was found associated with R. persica spores. In particular, sequences belonging to 13 phyla were mostly represented by Bacillota (46.6%), Pseudomonadota (39.7%), Actinomycetota (10.4%) and Bacteroidota (2%). Here, the two most abundant genera were represented by two endobacteria (45.4%), while the remaining sequences belonged to 87 different genera, the majority of which known as plant growth promoters. Culture-dependent approaches allowed the isolation of 203 and 81 bacterial strains from R. persica and R. fulgida spores, respectively. Interestingly, diverse bacterial communities were associated with the spores of the two AMF, although originated from the same host plants and environmental conditions, showing that each AMF isolate recruits on its spores a different microbiota. Such bacterial isolates will be further analysed for their functional properties, to be used as plant beneficial microbial inocula.
THE MICROBIOTA OF AMF SPORES ASSOCIATED WITH AMMOPHILA ARENARIA: A RICH SOURCE OF BENEFICIAL BACTERIA
Grassi A.Primo
;Agnolucci M.Secondo
;Turrini A.Ultimo
2023-01-01
Abstract
The aim of this study was the isolation and characterization the microbiota associated with the arbuscular mycorrhizal fungi (AMF) colonizing the roots of Ammophila arenaria. Rhizosphere soil from three A. arenaria plants was collected from a stable dune system in the Migliarino-San Rossore-Massaciuccoli Natural Park, on Tuscan coast. The dominant AMF species retrieved were Racocetra persica, Racocetra fulgida, Acaulospora scrobiculata, Rhizoglomus irregulare and Glomus spp. In particular, the genus Racocetra was the most abundant (68%) in A. arenaria rhizosphere. Illumina MiSeq sequencing of bacteria associated with R. fulgida spores allowed the detection of 9 phyla, mostly represented by Bacillota (89.9%), Actinomycetota (5.5%) and Pseudomonadota (3.4%). Interestingly, most sequences were identified as Mollicutes/mycoplasma-related endobacteria. A richer bacterial community was found associated with R. persica spores. In particular, sequences belonging to 13 phyla were mostly represented by Bacillota (46.6%), Pseudomonadota (39.7%), Actinomycetota (10.4%) and Bacteroidota (2%). Here, the two most abundant genera were represented by two endobacteria (45.4%), while the remaining sequences belonged to 87 different genera, the majority of which known as plant growth promoters. Culture-dependent approaches allowed the isolation of 203 and 81 bacterial strains from R. persica and R. fulgida spores, respectively. Interestingly, diverse bacterial communities were associated with the spores of the two AMF, although originated from the same host plants and environmental conditions, showing that each AMF isolate recruits on its spores a different microbiota. Such bacterial isolates will be further analysed for their functional properties, to be used as plant beneficial microbial inocula.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
