Aim of this study was to evaluate the effect of dietary vitamin E plus Selenium (seleno-L-methionine) supplementation on “in vitro” forward progressive movement of pheasant spermatozoa collected at the end of the reproductive season. Thirty common pheasants fed on a male breeder diet (E.M. 11.51 MJ/Kg, C.P. 19.0%) containing 1% fish oil, were divided into three dietary groups differing for the Se/vitamin E ratio: 0.0005, 0.0015 and 0.0025 (Se 0.1, 0.3 and 0.1 mg/kg of feed and Vit E 200, 200 and 40 mg/kg, respectively). After a one-month period of dietary treatment, semen was evaluated three times every 14 days. Semen of each dietary group was diluted 1:1 using the following extenders: commercial pheasant semen extender (IMV, L’Aigle, France), BPSE (Sexton, 1977) and Lake and Ravie (1979) diluent. Sperm concentration, mobility (Froman, 1997) and fatty acid composition were determined on fresh semen. No statistical difference was observed in sperm concentration between groups. Better mobility (P<0.01) was observed in spermatozoa from pheasants fed on diets with the two highest Se/vitamin E ratios. The 0.0015 Se/Vitamin E ratio diet permitted a lower proportion of saturated/unsaturated fatty acids, an increase in n-3 PUFAs and consequently a low n-6/n-3 ratio in spermatozoa.. Sperm mobility was not affected by diluents. The highest Se/vitamin E ratio diet gave good results, although its vitamin E content was the lowest. However, to better protect sperm membrane, 200mg vitamin E content is advisable together with at least 0.0015 Se/vitamin E ratio.

Effect of dietary selenium/vitamin E ratio on mobility of pheasant spermatozoa

MARZONI FECIA DI COSSATO, MARGHERITA;ROMBOLI, ISABELLA
2010

Abstract

Aim of this study was to evaluate the effect of dietary vitamin E plus Selenium (seleno-L-methionine) supplementation on “in vitro” forward progressive movement of pheasant spermatozoa collected at the end of the reproductive season. Thirty common pheasants fed on a male breeder diet (E.M. 11.51 MJ/Kg, C.P. 19.0%) containing 1% fish oil, were divided into three dietary groups differing for the Se/vitamin E ratio: 0.0005, 0.0015 and 0.0025 (Se 0.1, 0.3 and 0.1 mg/kg of feed and Vit E 200, 200 and 40 mg/kg, respectively). After a one-month period of dietary treatment, semen was evaluated three times every 14 days. Semen of each dietary group was diluted 1:1 using the following extenders: commercial pheasant semen extender (IMV, L’Aigle, France), BPSE (Sexton, 1977) and Lake and Ravie (1979) diluent. Sperm concentration, mobility (Froman, 1997) and fatty acid composition were determined on fresh semen. No statistical difference was observed in sperm concentration between groups. Better mobility (P<0.01) was observed in spermatozoa from pheasants fed on diets with the two highest Se/vitamin E ratios. The 0.0015 Se/Vitamin E ratio diet permitted a lower proportion of saturated/unsaturated fatty acids, an increase in n-3 PUFAs and consequently a low n-6/n-3 ratio in spermatozoa.. Sperm mobility was not affected by diluents. The highest Se/vitamin E ratio diet gave good results, although its vitamin E content was the lowest. However, to better protect sperm membrane, 200mg vitamin E content is advisable together with at least 0.0015 Se/vitamin E ratio.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/138745
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