Ceratocystis platani is the causal agent of canker stain, the most dangerous disease affecting the plane trees. The fungus produces cerato-platanin (CP), a protein of about 12.4 kDa acting as a PAMP in host and non-host plants. On plane leaves CP elicits the transcription of defence-related genes earlier than C. platani does. The amino acid sequence 1-119 of CP is a new protein domain, called "cerato-platanin domain”; thus, CP is the founding member of the cerato-platanin family (pfam PF07249). To date, a number of highly conserved proteins produced by Ascomycetes and Basidiomycetes have been identified with this domain. They have been reported to interact with plants and humans, but very little is known about the regulation of the genes coding for these proteins and about their primary role in the lifestyle of producing fungi. In the present work we demonstrated that CP is released by the fungus during the interaction with the host plant, and the expression of the cp gene is highly modulated. The gene was expressed more rapidly when the fungus was inoculated on the plane leaves than when it was grown in axenic culture. Potential abiotic and biotic stressors have been investigated: temperature, H2O2, umbelliferone (the reference plane phytoalexin), matric water stress, light (presence/absence), growth on sawdust of susceptible and resistant plane or elm trees, and co-culture with Trichoderma atroviride P1 and T. harzianum T22. The gene expression has been evaluated by qRT-PCR using TaqMan probes. The promoter region has been isolated and studied.
Is the expression of the gene coding for cerato-platanin modulated by biotic and abiotic factors?
BERNARDI, RODOLFO;
2010-01-01
Abstract
Ceratocystis platani is the causal agent of canker stain, the most dangerous disease affecting the plane trees. The fungus produces cerato-platanin (CP), a protein of about 12.4 kDa acting as a PAMP in host and non-host plants. On plane leaves CP elicits the transcription of defence-related genes earlier than C. platani does. The amino acid sequence 1-119 of CP is a new protein domain, called "cerato-platanin domain”; thus, CP is the founding member of the cerato-platanin family (pfam PF07249). To date, a number of highly conserved proteins produced by Ascomycetes and Basidiomycetes have been identified with this domain. They have been reported to interact with plants and humans, but very little is known about the regulation of the genes coding for these proteins and about their primary role in the lifestyle of producing fungi. In the present work we demonstrated that CP is released by the fungus during the interaction with the host plant, and the expression of the cp gene is highly modulated. The gene was expressed more rapidly when the fungus was inoculated on the plane leaves than when it was grown in axenic culture. Potential abiotic and biotic stressors have been investigated: temperature, H2O2, umbelliferone (the reference plane phytoalexin), matric water stress, light (presence/absence), growth on sawdust of susceptible and resistant plane or elm trees, and co-culture with Trichoderma atroviride P1 and T. harzianum T22. The gene expression has been evaluated by qRT-PCR using TaqMan probes. The promoter region has been isolated and studied.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
