Local sheep breeds from southern Italy have suffered in the last decades a severe population size decline due to replacement with selected breeds. Presently, interest in preserving these resources has grown up and attempts are being made to encourage farmers to rear native sheep breeds. Authentication and verification of the breed origin of sheep meat may represent a valuable tool of commercial valorization. This study aims to compare the statistical power in assigning sheep samples to their true bree (sarda and Comisana) prevalent on the national lamb market and five local rare sheep breeds from Southern Italy (Bagnolese, laticauda, Gentile di Puglia, Altamurana, Leccese) were type at 19 STR and 104 SNP loci. A Maximum Likelihood-based assignment test was adopted to evaluate the proportion of correct breed allocation.STR mrkers performed better than SNP markers in all the seven breeds (with an average percentage of corrct allocation equal to 99.6 +- 0.5 and 85.9 +- 15.5, respectively). Despite the low genetic differentiation among the considered breeds ( overall fst= 0.049), results suggest that there is enough room to optimize a molecular tool able to discriminate among the local sheep breed from southern Italy and the two national selected breeds.
Power analysis of a population assignment test via SNP and STR markers with a view to breed authentication of sheep meat from native Southern Italy breeds
CECCHI, FRANCESCA;CIAMPOLINI, ROBERTA
2010-01-01
Abstract
Local sheep breeds from southern Italy have suffered in the last decades a severe population size decline due to replacement with selected breeds. Presently, interest in preserving these resources has grown up and attempts are being made to encourage farmers to rear native sheep breeds. Authentication and verification of the breed origin of sheep meat may represent a valuable tool of commercial valorization. This study aims to compare the statistical power in assigning sheep samples to their true bree (sarda and Comisana) prevalent on the national lamb market and five local rare sheep breeds from Southern Italy (Bagnolese, laticauda, Gentile di Puglia, Altamurana, Leccese) were type at 19 STR and 104 SNP loci. A Maximum Likelihood-based assignment test was adopted to evaluate the proportion of correct breed allocation.STR mrkers performed better than SNP markers in all the seven breeds (with an average percentage of corrct allocation equal to 99.6 +- 0.5 and 85.9 +- 15.5, respectively). Despite the low genetic differentiation among the considered breeds ( overall fst= 0.049), results suggest that there is enough room to optimize a molecular tool able to discriminate among the local sheep breed from southern Italy and the two national selected breeds.File | Dimensione | Formato | |
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