"Sangiovese" is known in the pro¬duction area of Morellino di Scansano wine as "Morellino". Although use of this synonym is widely accepted by grapevine growers, no in-depth study on the population of this grapevine variety has been undertaken to date in this area. Research on clonal selection of "Sangiovese" conducted in the framework of the "Qualitative Improvement of Gra-pevine Production and Propagation Material" project within the above-specified area also focused on a biotype with special characteristics, known locally as "Morellino pizzuto". Investigations were car¬ried out to improve knowledge of the ampelographic and genetic charac¬teristics and health status of this variety. Ampelographic determinations on various plant parts of two presumed clones (CH6 and GA5) led to identification of characteristics distinguishing these from the rest of the "Sangiovese" population. In particular, the two presumed clones were distinguished from the homologated clones "Sangiovese "RIO" and "SS-F9-A5-48" used as a reference. The relevant characters were mainly apex and lower leaf surface tomentosity, intensity of shoot tip anthocyanin pigmentation and of lower leaf surface venation, cluster and grape shape. The two presumed clones were compared with "Sangiovese" clones "RIO", "SS-F9-A5-48" and "BBS II" using the microsatellite amplification test, in order to assess their putative genetic identity. DNA extracted from leaf material of container-grown samples was purified and amplified using the PCR technique. Analysis of several loci showed that the biotype of "Mo¬rellino pizzuto" presents certain differences in comparison to traditional "Sangiovese" but also has some degree of relationship with the latter. The grapevines sanitary sta¬tus was determined by immunoenzymatic assays (DAS e TAS-ELISA) and indexing onto Vitis spp. indicators. The results showed that the material examined was free from grapevine fanleaf nepovirus (GFLV), grapevine leafroll complex (GLRV), grapevine rugose wood complex (RW) and grapevine fleck virus (GFkV). Possible presence of virus-like diseases was investigated by repeated field inspections. Results ruled out the presence of symptoms ascribable to phytoplasma infections and to grapevine enation disease. Negative results for the latter were also obtained through indexing on hybrid LN 33. Presence of grapevine vein necrosis disease (GVND), on only one of the presumed clones, was ascertained by indexing on 110 R. Overall, results obtained suggest that the differences observed in com¬paring these two presumed clones with the reference germplasm cannot be attributed to viral or viral-like infection states
Il "Morellino Pizzuto": un biotipo di "Sangiovese"?
SCALABRELLI, GIANCARLO;MATERAZZI, ALBERTO;TRIOLO, ENRICO
2001-01-01
Abstract
"Sangiovese" is known in the pro¬duction area of Morellino di Scansano wine as "Morellino". Although use of this synonym is widely accepted by grapevine growers, no in-depth study on the population of this grapevine variety has been undertaken to date in this area. Research on clonal selection of "Sangiovese" conducted in the framework of the "Qualitative Improvement of Gra-pevine Production and Propagation Material" project within the above-specified area also focused on a biotype with special characteristics, known locally as "Morellino pizzuto". Investigations were car¬ried out to improve knowledge of the ampelographic and genetic charac¬teristics and health status of this variety. Ampelographic determinations on various plant parts of two presumed clones (CH6 and GA5) led to identification of characteristics distinguishing these from the rest of the "Sangiovese" population. In particular, the two presumed clones were distinguished from the homologated clones "Sangiovese "RIO" and "SS-F9-A5-48" used as a reference. The relevant characters were mainly apex and lower leaf surface tomentosity, intensity of shoot tip anthocyanin pigmentation and of lower leaf surface venation, cluster and grape shape. The two presumed clones were compared with "Sangiovese" clones "RIO", "SS-F9-A5-48" and "BBS II" using the microsatellite amplification test, in order to assess their putative genetic identity. DNA extracted from leaf material of container-grown samples was purified and amplified using the PCR technique. Analysis of several loci showed that the biotype of "Mo¬rellino pizzuto" presents certain differences in comparison to traditional "Sangiovese" but also has some degree of relationship with the latter. The grapevines sanitary sta¬tus was determined by immunoenzymatic assays (DAS e TAS-ELISA) and indexing onto Vitis spp. indicators. The results showed that the material examined was free from grapevine fanleaf nepovirus (GFLV), grapevine leafroll complex (GLRV), grapevine rugose wood complex (RW) and grapevine fleck virus (GFkV). Possible presence of virus-like diseases was investigated by repeated field inspections. Results ruled out the presence of symptoms ascribable to phytoplasma infections and to grapevine enation disease. Negative results for the latter were also obtained through indexing on hybrid LN 33. Presence of grapevine vein necrosis disease (GVND), on only one of the presumed clones, was ascertained by indexing on 110 R. Overall, results obtained suggest that the differences observed in com¬paring these two presumed clones with the reference germplasm cannot be attributed to viral or viral-like infection statesI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
