AIM. To evaluate: 1) repeatability in BALF WBC count; 2) turbidity evaluation of BALF for mucus quantification. MATERIALS AND METHODS. BAL was performed in 31 healthy trotter mare aged 3-6 years. Tracheal mucus was quantified by a scoring system (0-5). BALF was evaluated 3 times consecutively by a cell counter for WBC and by a spectrophotometer for turbidity (DO). ANOVA test was performed to verify differences between the 3 evaluations, both for WBC and DO. Statistical differences were set at p<0.05. Average of the 3 DO was calculated and a correlation was verify between DO average and mucus score. RESULTS. No statistical differences were obtained for ANOVA test, while a statistical significative correlation (r: 0,82) was obtained for DO vs mucus score. DISCUSSION AND CONCLUSION – Our results confirm the repeatability of WBC evaluation by a cell counter and the useful of turbidity evaluation of BALF with a spectrophotometer for mucus quantification.AIM. To evaluate: 1) repeatability in BALF WBC count; 2) turbidity evaluation of BALF for mucus quantification. MATERIALS AND METHODS. BAL was performed in 31 healthy trotter mare aged 3-6 years. Tracheal mucus was quantified by a scoring system (0-5). BALF was evaluated 3 times consecutively by a cell counter for WBC and by a spectrophotometer for turbidity (DO). ANOVA test was performed to verify differences between the 3 evaluations, both for WBC and DO. Statistical differences were set at p<0.05. Average of the 3 DO was calculated and a correlation was verify between DO average and mucus score. RESULTS. No statistical differences were obtained for ANOVA test, while a statistical significative correlation (r: 0,82) was obtained for DO vs mucus score. DISCUSSION AND CONCLUSION – Our results confirm the repeatability of WBC evaluation by a cell counter and the useful of turbidity evaluation of BALF with a spectrophotometer for mucus quantification.