Cerato-platanin (CP) is a small protein produced by Ascomycete Ceratocystis platani, the causal agent of plane canker stain. Cep is pathogenic to Platanus orientalis, P. occidentalis and their hybrid P. acerifolia. CP is located in the fungal cell walls, is early released in culture, and elicits defence-related structural and physiological responses in host and non-host plants, such as cell plasmolysis and death, phenolic compounds and phytoalexin accumulation (Pazzagli et al., J. Biol. Chem. 274: 24959-24964, 1999; Boddi et al., FEMS Microbiol. Letters 233: 341-346, 2004; Scala et al., J. Plant Pathol. 86: 23-29,2004; Bennici et al., Caryologia 59: 291-298, 2006). Recently, it has been demonstrated the close correlation between the CP treatment of plane leaves and the subsequent growth inhibition of C. platani on the surface of the treated leaves together with the production of phytoalexins and the over-transcription of regulatory and defense-associated genes (Fontana et al., J. Plant Pathol. 90: 293-304, 2008). The aim of the present study was to provide further information on the modulation of gene expression that occurs in the plane tree by CP treatment in order to improve our understanding of the potential of CP to function as a PAMP (Pathogen-Associated Molecular Pattern) protein in the pathogenic process of the C. platani-P. acerifolia interaction. In the present study we have isolated others clones from a cDNA library constructed using suppression subtractive hybridization (SSH) technique (Fontana et al., J. Plant Pathol. 90: 293-304, 2008) and the putative differentially expressed genes were identified using the FASTA, BLASTN and BLASTX programs. The up-regulated clones were classified in the macro putative groups taking in account the functional categories established for Arabidopsis (The Arabidopsis Genome Initiative, Nature 408: 796-815, 2000). We have analysed, moreover, some of them by relative PCR using total RNA extracted from leaves treated with CP or fungal conidia at 6, 24 and 48 hours after treatments in order to investigate possible differences in gene expression during CP/plant and fungus/plant interactions.

Modulation of Gene Expression Induced in Platanus Acerifolia by Cerato-Platanin and Conidial Suspension of Ceratocystis Platani

BERNARDI, RODOLFO
2008

Abstract

Cerato-platanin (CP) is a small protein produced by Ascomycete Ceratocystis platani, the causal agent of plane canker stain. Cep is pathogenic to Platanus orientalis, P. occidentalis and their hybrid P. acerifolia. CP is located in the fungal cell walls, is early released in culture, and elicits defence-related structural and physiological responses in host and non-host plants, such as cell plasmolysis and death, phenolic compounds and phytoalexin accumulation (Pazzagli et al., J. Biol. Chem. 274: 24959-24964, 1999; Boddi et al., FEMS Microbiol. Letters 233: 341-346, 2004; Scala et al., J. Plant Pathol. 86: 23-29,2004; Bennici et al., Caryologia 59: 291-298, 2006). Recently, it has been demonstrated the close correlation between the CP treatment of plane leaves and the subsequent growth inhibition of C. platani on the surface of the treated leaves together with the production of phytoalexins and the over-transcription of regulatory and defense-associated genes (Fontana et al., J. Plant Pathol. 90: 293-304, 2008). The aim of the present study was to provide further information on the modulation of gene expression that occurs in the plane tree by CP treatment in order to improve our understanding of the potential of CP to function as a PAMP (Pathogen-Associated Molecular Pattern) protein in the pathogenic process of the C. platani-P. acerifolia interaction. In the present study we have isolated others clones from a cDNA library constructed using suppression subtractive hybridization (SSH) technique (Fontana et al., J. Plant Pathol. 90: 293-304, 2008) and the putative differentially expressed genes were identified using the FASTA, BLASTN and BLASTX programs. The up-regulated clones were classified in the macro putative groups taking in account the functional categories established for Arabidopsis (The Arabidopsis Genome Initiative, Nature 408: 796-815, 2000). We have analysed, moreover, some of them by relative PCR using total RNA extracted from leaves treated with CP or fungal conidia at 6, 24 and 48 hours after treatments in order to investigate possible differences in gene expression during CP/plant and fungus/plant interactions.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/198232
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