Cerato-platanin (CP) and cerato-populin (Pop1) are small proteins produced by the phytopathogenic fungi Ceratocystis platani and C. populicola, respectively. CP and Pop1 behave as PAMPs, since they elicit typical defense responses in various host and non-host plants such as the induction of synthesis of phytoalexins and of some defence related genes. CP structure has been solved by NMR and consists in a double Ψbarrel protein. Pop1 has a similar fold but circular dichroism shows differences in the secondary structure between the two proteins. In order to better characterize the PAMP activity of these proteins we first assayed: (i) the production of hydrogen peroxide and nitric oxide; (ii) the viability of cells; (iii) the expression of the genes PR5 (thaumatin), LTP (lipid transfer protein) e APX (ascorbate peroxidase). Results indicate that either CP and Pop1 induce synthesis of H2O2, NO and hypersensitive cell death with apoptotic features which are hallmark of successful recognition of infection and activation of plant defences. Recently, the activation of MAPKs has been detected in Arabidopsis and plane leaves treated with 1.5 nmol/10 μl of CP and Pop1. After 15 min and 1, 3, 6, 12, 24 and 48 h of incubation, leaves were subjected to protein extraction, and sample were analyzed by Western blot using antierk1/2 human antibodies. Preliminary results show the activation of MAPK 3/6 after 1-12 h of incubation. Therefore, another marker of defence response was shown to be activated by CP and Pop1 in specific and non-specific hosts.

Cerato-platanin and cerato-populin induce mapk activation in plane and Arabidopsis leaves.

LOMBARDI, LARA;BERNARDI, RODOLFO;PICCIARELLI, PIERO;
2012

Abstract

Cerato-platanin (CP) and cerato-populin (Pop1) are small proteins produced by the phytopathogenic fungi Ceratocystis platani and C. populicola, respectively. CP and Pop1 behave as PAMPs, since they elicit typical defense responses in various host and non-host plants such as the induction of synthesis of phytoalexins and of some defence related genes. CP structure has been solved by NMR and consists in a double Ψbarrel protein. Pop1 has a similar fold but circular dichroism shows differences in the secondary structure between the two proteins. In order to better characterize the PAMP activity of these proteins we first assayed: (i) the production of hydrogen peroxide and nitric oxide; (ii) the viability of cells; (iii) the expression of the genes PR5 (thaumatin), LTP (lipid transfer protein) e APX (ascorbate peroxidase). Results indicate that either CP and Pop1 induce synthesis of H2O2, NO and hypersensitive cell death with apoptotic features which are hallmark of successful recognition of infection and activation of plant defences. Recently, the activation of MAPKs has been detected in Arabidopsis and plane leaves treated with 1.5 nmol/10 μl of CP and Pop1. After 15 min and 1, 3, 6, 12, 24 and 48 h of incubation, leaves were subjected to protein extraction, and sample were analyzed by Western blot using antierk1/2 human antibodies. Preliminary results show the activation of MAPK 3/6 after 1-12 h of incubation. Therefore, another marker of defence response was shown to be activated by CP and Pop1 in specific and non-specific hosts.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11568/227727
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