Cerato-platanin (CP) is a non-catalytic protein with a doubleoa-barrel fold located in the cell wall of the phytopathogenic fungus Ceratocystis platani. CP is the first member of a protein family named the “cerato-platanin family” (Pfam PF07249), which comprises proteins produced by plant pathogenic and non-pathogenic fungi. Some of these proteins show resistance-inducing activity in plants. Their biological role in fungal life remains unknown, however concerning CP we have recently hypothesised a dual role: a structural role in the fungal cell wall due to the ability to bind chitin, and a role in the fungus-plant interaction due to the ability to weaken cellulosic materials.CP induces defence-related responses when applied on host and non-host plants. In plane tree (Platanus acerifolia) leaves these responses have been extensively studied.In the present study we show that CP triggers resistance in Arabidopsis leaves: CP quickly induced the synthesis of hydrogen peroxide at the level of stomata, the synthesis of nitric oxide, it caused stomatal closure and overexpression of salicylic acid- and ethylene-dependent genes and it induced the synthesis of camalexin. After 24h of surface treatment with CP, Arabidopsis leaves showed reduced susceptibility to Botrytis cinerea similarly to that occurred by using chitosan. This is the first report of a resistance-inducing activity by CP against a pathogen able to colonise leaves.We are currently investigating the mechanism by which CP is perceived by the plant leaf surface and the ability of this protein to protect Arabidopsis leaves against Pseudomonas syringae pv. tomato.

The fungal MAMP cerato-platanin triggers resistance against Botrytis cinerea in Arabidopsis leaves

LOMBARDI, LARA;BERNARDI, RODOLFO;PICCIARELLI, PIERO;
2013-01-01

Abstract

Cerato-platanin (CP) is a non-catalytic protein with a doubleoa-barrel fold located in the cell wall of the phytopathogenic fungus Ceratocystis platani. CP is the first member of a protein family named the “cerato-platanin family” (Pfam PF07249), which comprises proteins produced by plant pathogenic and non-pathogenic fungi. Some of these proteins show resistance-inducing activity in plants. Their biological role in fungal life remains unknown, however concerning CP we have recently hypothesised a dual role: a structural role in the fungal cell wall due to the ability to bind chitin, and a role in the fungus-plant interaction due to the ability to weaken cellulosic materials.CP induces defence-related responses when applied on host and non-host plants. In plane tree (Platanus acerifolia) leaves these responses have been extensively studied.In the present study we show that CP triggers resistance in Arabidopsis leaves: CP quickly induced the synthesis of hydrogen peroxide at the level of stomata, the synthesis of nitric oxide, it caused stomatal closure and overexpression of salicylic acid- and ethylene-dependent genes and it induced the synthesis of camalexin. After 24h of surface treatment with CP, Arabidopsis leaves showed reduced susceptibility to Botrytis cinerea similarly to that occurred by using chitosan. This is the first report of a resistance-inducing activity by CP against a pathogen able to colonise leaves.We are currently investigating the mechanism by which CP is perceived by the plant leaf surface and the ability of this protein to protect Arabidopsis leaves against Pseudomonas syringae pv. tomato.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/471667
 Attenzione

Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo

Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact