TT virus (TTV) loads associated with the peripheral blood cells of seven patients known to carry the virus in plasma were investigated by real-time PCR. Whereas red cells/platelets were uniformly negative, six and four patients yielded positive peripheral blood mononuclear cells (PBMCs) and polymorphonuclear leukocytes, respectively, but viral titres were generally low. Fractionation of PBMCs into monocyte- and B, T4, and T8 lymphocyte-enriched subpopulations showed no pattern in the viral loads that might suggest the preferential association of TTV to one or more specific cell types. TTV-negative PBMCs absorbed measurable amounts of virus when incubated with infected plasma at 4 degreesC. Furthermore, cultures of TTV-negative phytohaemagglutinin-stimulated PBMCs exposed in vitro to virus-positive plasma and faecal extracts released considerable levels of infectious TTV into the supernatant fluid and the same was true for TTV-positive stimulated PBMCs. These results indicate that, whereas freshly harvested resting PBMCs seem to produce little, if any TTV, stimulated PBMCs actively replicate the virus. (C) 2001 Wiley-Liss, Inc.
|Autori interni:||PISTELLO, MAURO|
|Autori:||Maggi F; Fornai C; Zaccaro L; Morrica A; Vatteroni ML; Isola P; Marchi S; Ricchiuti A; Pistello M; Bendinelli R|
|Titolo:||TT virus (TTV) leads associated with different peripheral blood cell types and evidence for TTV replication in activated mononuclear cells|
|Anno del prodotto:||2001|
|Digital Object Identifier (DOI):||10.1002/jmv.1035|
|Appare nelle tipologie:||1.1 Articolo in rivista|