In this work, three biotypes (‘Br’, ‘Co’ ed ‘Ru’) of ‘Durella’ found in Lunigiana area (MS) were separated by ampelographic and microsatellite analysis from three homologated clones (ISV CVI 4, ISV CVI 8, ISV CVI 13) of ‘Durella’ cultivated in Vicenza province. The cluster analysis of ampelographic observations (according Eu-Project, Genres, 1997) permitted to distinguish the biotypes of ‘Durella’ coming from Lunigiana from the homologated clones of ‘Durella’. Within the ‘Durella’ of Lunigiana group, the biotype ‘Br’ showed some differences from the biotypes ‘Co’ and ‘Ru’. Also the microsatellite analysis carried out in five different loci (VVS4, VVMD14, VVMD17, VVMD21, VVMD24) allow us to distinguish the biotypes of ‘Durella’ of Lunigiana from the homologated clones of ‘Durella’ coming from Vicenza province. The biotypes ‘Co’ and ‘Ru’ were identical in all the analysed loci, whereas the biotype ‘Br’ showed different allele size on 4 loci. The three homolated clones were identical in the five loci tested. The cluster analysis performed on allelic size indicated that the biotype ‘Br’ had more similarity to the ‘Durella’ of Lunigiana group. A possible parentage between the two ‘Durella’ groups is suggested by the presence of at least one identical allelic size in the five analysed microsatellite loci.
Caratterizzazione varietale assistita con marcatori molecolari delle Durelle autoctone della Lunigiana
D'ONOFRIO, CLAUDIO;SCALABRELLI, GIANCARLO;
2002-01-01
Abstract
In this work, three biotypes (‘Br’, ‘Co’ ed ‘Ru’) of ‘Durella’ found in Lunigiana area (MS) were separated by ampelographic and microsatellite analysis from three homologated clones (ISV CVI 4, ISV CVI 8, ISV CVI 13) of ‘Durella’ cultivated in Vicenza province. The cluster analysis of ampelographic observations (according Eu-Project, Genres, 1997) permitted to distinguish the biotypes of ‘Durella’ coming from Lunigiana from the homologated clones of ‘Durella’. Within the ‘Durella’ of Lunigiana group, the biotype ‘Br’ showed some differences from the biotypes ‘Co’ and ‘Ru’. Also the microsatellite analysis carried out in five different loci (VVS4, VVMD14, VVMD17, VVMD21, VVMD24) allow us to distinguish the biotypes of ‘Durella’ of Lunigiana from the homologated clones of ‘Durella’ coming from Vicenza province. The biotypes ‘Co’ and ‘Ru’ were identical in all the analysed loci, whereas the biotype ‘Br’ showed different allele size on 4 loci. The three homolated clones were identical in the five loci tested. The cluster analysis performed on allelic size indicated that the biotype ‘Br’ had more similarity to the ‘Durella’ of Lunigiana group. A possible parentage between the two ‘Durella’ groups is suggested by the presence of at least one identical allelic size in the five analysed microsatellite loci.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
