Cerato-platanin (CP) is a 12.4 kDa protein purified from the culture filtrate of Ceratocystis fimbriata f. sp. platani (Cfp), the causal agent of the plane canker stain (Pazzagli et al., 1999, J Biol Chem 274: 24959-24964). CP is 120 amino acids in length, contains 4 cysteines forming two S-S bridges, Cys20-57 and Cys60-115, and has a high percentage (40%) of hydrophobic residues. According to the data banks, CP is the founder member of the "cerato-platanin family" and shares some of its chemical and physical characteristics with the hydrophobins and with the few other hydrophobic cell wall proteins. CP is located in the Cfp cell walls, is able to self-aggregate and elicits defense responses in host and in non-host plant tissues (Bennici et al, 2005, Phytopathol Medit 44: in press; Boddi et al, 2004, FEMS Microbiol Letters 233: 341-346, Pazzagli et al, 2005, Cell Biochem Biophys 42: in press; Scala et al. 2004, J Plant Pathol 86: 23-29). In order to understand the role of CP in pathogenesis a multidisciplinary approach has been undertaken, and some of the studies in progress concern: (1) the isolation of genes hortologous to CP; (2) the determination of the three-dimensional structure of CP; (3) the aggregation of cerato-platanin, and its effect on plane cells; (4) the characterisation of Cfp mutants CP non-producers; (5) the screening of two Suppressive Subtractive Hybridisation (SSH) cDNA libraries constructed from RNAs extracted from plane leaves treated with CP or Cfp; (6) the regulation of the isolated gene sequences.

A multidisciplinary approach to study the role of cerato-platanin, a small protein from Ceratocystis fimbriata f. sp. platani, in the pathogenesis of the plane canker stain

BERNARDI, RODOLFO;
2005

Abstract

Cerato-platanin (CP) is a 12.4 kDa protein purified from the culture filtrate of Ceratocystis fimbriata f. sp. platani (Cfp), the causal agent of the plane canker stain (Pazzagli et al., 1999, J Biol Chem 274: 24959-24964). CP is 120 amino acids in length, contains 4 cysteines forming two S-S bridges, Cys20-57 and Cys60-115, and has a high percentage (40%) of hydrophobic residues. According to the data banks, CP is the founder member of the "cerato-platanin family" and shares some of its chemical and physical characteristics with the hydrophobins and with the few other hydrophobic cell wall proteins. CP is located in the Cfp cell walls, is able to self-aggregate and elicits defense responses in host and in non-host plant tissues (Bennici et al, 2005, Phytopathol Medit 44: in press; Boddi et al, 2004, FEMS Microbiol Letters 233: 341-346, Pazzagli et al, 2005, Cell Biochem Biophys 42: in press; Scala et al. 2004, J Plant Pathol 86: 23-29). In order to understand the role of CP in pathogenesis a multidisciplinary approach has been undertaken, and some of the studies in progress concern: (1) the isolation of genes hortologous to CP; (2) the determination of the three-dimensional structure of CP; (3) the aggregation of cerato-platanin, and its effect on plane cells; (4) the characterisation of Cfp mutants CP non-producers; (5) the screening of two Suppressive Subtractive Hybridisation (SSH) cDNA libraries constructed from RNAs extracted from plane leaves treated with CP or Cfp; (6) the regulation of the isolated gene sequences.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/99292
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