Resequencing in resistant cultivar ‘Nure’ and structural comparison with the same region of susceptible ‘Morex’ was performed in order to gain a better insight into barley Frost-resistance-H2 locus. Accurate annotation showed copy number variation (CNV) in the proximal part of the locus. In ‘Nure’, two exact copies of the HvCBF4- HvCBF2A region and one of the HvCBF4-HvCBF2B segment were observed, while in ‘Morex’ the corresponding region harboured a single HvCBF4-HvCBF2A (22 kb) segment. Abundance and diversity of repetitive element classes, gene function gain/losses, regulatory motifs and SNPs in gene sequences were identified. An expression study of key HvCBFs with/without CNV on selected genotypes contrasting for frost resistance and estimated HvCBF4-HvCBF2B copy number (2–10 copies) was also performed. Under light stimulus at warm temperature (23 °C), CNV of HvCBF2A and HvCBF4 correlated with their expression levels and reported frost resistance of genotypes; moreover, expression levels of HvCBF2A and HvCBF14 were strongly correlated (r=0.908, p < 0.01). On the other hand, frost resistance correlated to HvCBF14 expression (r=0.871, p < 0.01) only after cold induction (6°C) in the dark. A complex interplay of HvCBFs expression levels under different light/ temperature stimuli is discussed in light of CNV and presence/number of regulatory elements that integrate different signal transduction pathways.
Influence of CNV on transcript levels of HvCBF genes at Fr-H2 locus revealed by resequencing in resistant barley cv. ‘Nure’ and expression analysis
Mascagni F.;Natali L.;Cavallini A.;
2020-01-01
Abstract
Resequencing in resistant cultivar ‘Nure’ and structural comparison with the same region of susceptible ‘Morex’ was performed in order to gain a better insight into barley Frost-resistance-H2 locus. Accurate annotation showed copy number variation (CNV) in the proximal part of the locus. In ‘Nure’, two exact copies of the HvCBF4- HvCBF2A region and one of the HvCBF4-HvCBF2B segment were observed, while in ‘Morex’ the corresponding region harboured a single HvCBF4-HvCBF2A (22 kb) segment. Abundance and diversity of repetitive element classes, gene function gain/losses, regulatory motifs and SNPs in gene sequences were identified. An expression study of key HvCBFs with/without CNV on selected genotypes contrasting for frost resistance and estimated HvCBF4-HvCBF2B copy number (2–10 copies) was also performed. Under light stimulus at warm temperature (23 °C), CNV of HvCBF2A and HvCBF4 correlated with their expression levels and reported frost resistance of genotypes; moreover, expression levels of HvCBF2A and HvCBF14 were strongly correlated (r=0.908, p < 0.01). On the other hand, frost resistance correlated to HvCBF14 expression (r=0.871, p < 0.01) only after cold induction (6°C) in the dark. A complex interplay of HvCBFs expression levels under different light/ temperature stimuli is discussed in light of CNV and presence/number of regulatory elements that integrate different signal transduction pathways.File | Dimensione | Formato | |
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