Renal failure is associated with disorders of vitamin E and vitamin A status [1,2]. Human patients on chronic haemodialysis tend to present deficiency of plasma vitamin E [3] and increase of vitamin A [4]. No data concerning the status of vitamin E and A are currently present in dogs submitted to haemodialysis. The aim of the present study was to evaluate the plasma concentration of vitamin E and vitamin A pre and post treatment in a group of dogs affected by acute and chronic kidney disease and submitted to intermittent haemodialysis (IHD). 30 dogs presented at the Service of Haemodialysis and Blood Purification of the University of Pisa, between September 2015 and April 2017, for acute kidney injury (AKI) and acute impairment of chronic kidney disease (AKI on CKD), and submitted to IHD were included. All patients underwent physical exam, abdominal ultrasound, renal panel and complete urinalysis. Plasma vitamin E and vitamin A were assessed through high performance liquid chromatography (HPLC) prior to haemodialysis and immediately after the treatment. Data were statistically analysed through GraphPad prism. Results were considered statistically significant for p<0.05. The study group was composed by 17 females and 13 males, of different breed, age and body weight. 7/30 dogs were diagnosed with AKI on CKD and 23/30 were diagnosed with AKI. The median pre-dialysis plasma concentration of vitamin E was 1.94 ppm (0.1-51.73 ppm); while the post-dialysis plasma concentration of vitamin E was 3.47 ppm (0.15-37.80 ppm). For vitamin A, the mean pre-dialysis plasma concentration was 0.58 ppm (±SD 0.30 ppm); while the post-dialysis plasma concentration was 0.55 ppm (±SD 0.30 ppm). No significant difference was found between pre-and post-dialysis plasma concentration of both vitamin E and vitamin A. Plasma concentration of vitamin E reduced significantly (p=0.03) from haemodialysis one to three. No significant difference in the number of dogs presenting reduction, increase or no variation in the plasma concentration of both vitamins, between pre-and post-dialysis treatment, was found. No significant correlation between plasma levels of vitamin E and vitamin A and plasma creatinine and urea was found. In the present study, the plasma concentrations of both vitamin E and vitamin A were lower than previously reported for azotemic dogs. Particularly, vitamin E deficiency seemed to worse with thenumber of dialysis. However, these plasma concentrations did not seem to be significantly affected by the hemodialysis treatment.
EFFECTS OF INTERMITTENT HAEMODIALYSIS ON PLASMA VITAMIN E AND VITAMIN A IN NEPHROPATIC DOGS
Ilaria Lippi
;Valentina Meucci;Grazia Guidi
2018-01-01
Abstract
Renal failure is associated with disorders of vitamin E and vitamin A status [1,2]. Human patients on chronic haemodialysis tend to present deficiency of plasma vitamin E [3] and increase of vitamin A [4]. No data concerning the status of vitamin E and A are currently present in dogs submitted to haemodialysis. The aim of the present study was to evaluate the plasma concentration of vitamin E and vitamin A pre and post treatment in a group of dogs affected by acute and chronic kidney disease and submitted to intermittent haemodialysis (IHD). 30 dogs presented at the Service of Haemodialysis and Blood Purification of the University of Pisa, between September 2015 and April 2017, for acute kidney injury (AKI) and acute impairment of chronic kidney disease (AKI on CKD), and submitted to IHD were included. All patients underwent physical exam, abdominal ultrasound, renal panel and complete urinalysis. Plasma vitamin E and vitamin A were assessed through high performance liquid chromatography (HPLC) prior to haemodialysis and immediately after the treatment. Data were statistically analysed through GraphPad prism. Results were considered statistically significant for p<0.05. The study group was composed by 17 females and 13 males, of different breed, age and body weight. 7/30 dogs were diagnosed with AKI on CKD and 23/30 were diagnosed with AKI. The median pre-dialysis plasma concentration of vitamin E was 1.94 ppm (0.1-51.73 ppm); while the post-dialysis plasma concentration of vitamin E was 3.47 ppm (0.15-37.80 ppm). For vitamin A, the mean pre-dialysis plasma concentration was 0.58 ppm (±SD 0.30 ppm); while the post-dialysis plasma concentration was 0.55 ppm (±SD 0.30 ppm). No significant difference was found between pre-and post-dialysis plasma concentration of both vitamin E and vitamin A. Plasma concentration of vitamin E reduced significantly (p=0.03) from haemodialysis one to three. No significant difference in the number of dogs presenting reduction, increase or no variation in the plasma concentration of both vitamins, between pre-and post-dialysis treatment, was found. No significant correlation between plasma levels of vitamin E and vitamin A and plasma creatinine and urea was found. In the present study, the plasma concentrations of both vitamin E and vitamin A were lower than previously reported for azotemic dogs. Particularly, vitamin E deficiency seemed to worse with thenumber of dialysis. However, these plasma concentrations did not seem to be significantly affected by the hemodialysis treatment.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
