Eustrongylides spp. are cosmopolitan parasitic nematodes with several freshwater fish species as intermediate or paratenic hosts, rarely reported as zoonotic agents. This work aims to report, for the first time, the occurrence of this parasite in specimens of big-scale sand smelt (Atherina boyeri) caught in the lake Massaciuccoli (Northwest Tuscany, Italy). Totally 3317 specimens of A. boyeri were collected in three different samplings (August–October 2019). Fish were visually examined and then submitted to artificial digestion. Visible parasites were collected and counted after both procedures, then they were identified to genus level by microscopic examination and to species level by molecular analysis of the ITS gene region. Overall, 75 nematodes identified as larval stages of Eustrongylides excisus were found (P: 2.3% 95% CI 1.8–2.8; MA: 0.02; MI: 1), in the viscera and in the muscle. A. boyeri is a highly appreciated fish species in several Italian areas as well as in various Mediterranean countries. Beside an epidemiological interest due to its role in the parasite cycle, the report of E. excisus in the study area arises possible quality and public health issues. Therefore, also considering the method of preparation of A. boyeri and that lately the increasing consumption of raw fish has also targeted freshwater fish species, it is essential to communicate the presence of this hazard and the need of applying appropriate sanitary measures to all FBOs along the supply chain and to consumers.
First detection of Eustrongylides excisus (Nematoda: Dioctophymatidae) in big-scale sand smelt (Atherina boyeri) from the lake Massaciuccoli (Northwest Tuscany, Italy): implications for public health and seafood quality
Armani A.Ultimo
2021-01-01
Abstract
Eustrongylides spp. are cosmopolitan parasitic nematodes with several freshwater fish species as intermediate or paratenic hosts, rarely reported as zoonotic agents. This work aims to report, for the first time, the occurrence of this parasite in specimens of big-scale sand smelt (Atherina boyeri) caught in the lake Massaciuccoli (Northwest Tuscany, Italy). Totally 3317 specimens of A. boyeri were collected in three different samplings (August–October 2019). Fish were visually examined and then submitted to artificial digestion. Visible parasites were collected and counted after both procedures, then they were identified to genus level by microscopic examination and to species level by molecular analysis of the ITS gene region. Overall, 75 nematodes identified as larval stages of Eustrongylides excisus were found (P: 2.3% 95% CI 1.8–2.8; MA: 0.02; MI: 1), in the viscera and in the muscle. A. boyeri is a highly appreciated fish species in several Italian areas as well as in various Mediterranean countries. Beside an epidemiological interest due to its role in the parasite cycle, the report of E. excisus in the study area arises possible quality and public health issues. Therefore, also considering the method of preparation of A. boyeri and that lately the increasing consumption of raw fish has also targeted freshwater fish species, it is essential to communicate the presence of this hazard and the need of applying appropriate sanitary measures to all FBOs along the supply chain and to consumers.File | Dimensione | Formato | |
---|---|---|---|
FOODCONT-D-20-01531R1.pdf
solo utenti autorizzati
Descrizione: Revised manuscript with cover letter and responses to reviewers
Tipologia:
Documento in Post-print
Licenza:
NON PUBBLICO - Accesso privato/ristretto
Dimensione
2.77 MB
Formato
Adobe PDF
|
2.77 MB | Adobe PDF | Visualizza/Apri Richiedi una copia |
Revised manuscript 1053118.pdf
Open Access dal 01/03/2022
Tipologia:
Documento in Post-print
Licenza:
Tutti i diritti riservati (All rights reserved)
Dimensione
2.78 MB
Formato
Adobe PDF
|
2.78 MB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.