Essential oils (EO) represent an important source of bioactive molecules and are widely used for their great efficacy relating to their different therapeutic properties. The present study aims to contribute to the valorization of Daucus virgatus (Poir.) Maire, an aromatic and native plant spontaneously growing in the North-Eastern regions of Algeria, by analyzing the chemical composition of its essential oil and evaluating the antioxidant and antimicrobial activities, together with the assay of the enzyme inhibitory effects against alpha-glucosidase and cholinesterase. Daucus virgatus EO was extracted by hydrodistillation and analyzed by gas chromatography-mass spectroscometry analysis (GC-MS). Twenty-one constituents accounting for 98% of the whole components were identified. beta-Pinene (77.9%) and alpha-pinene (7.6%) were the most abundant components. The antimicrobial activity against the pathogen microorganisms Listeria innocua, Salmonella typhimurium, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli and Candida albicans was investigated. The best antimicrobial effect was observed against S. typhimurium and C. albicans, which showed high sensitivity, with inhibition zones ranging between 27.3 and 25 mm; the minimum inhibitory concentrations were 15.63 and 31.25 mu g ml(-1), respectively. In the DPPH test, the essential oil showed a moderate antioxidant effect, with an IC50 value of 39.61 mg ml(-1). D. virgatus EO exhibited an interesting inhibitory effect against alpha-glucosidase enzyme (IC50 = 0.35 mg ml(-1)) compared with the positive control Acarbose (IC50 = 0.24 mg ml(-1)), and a moderate inhibitory effect against cholinesterases enzymes, with IC50 values of 0.33 and 0.20 mg ml(-1) for acetylcholinesterase and butyrylcholinesterase, respectively. D. virgatus EO might be used as a promising source of natural products with potential antimicrobial, antihyperglycemic and anti-Alzheimer effects.
Chemical Analysis, Antioxidant, Antimicrobial and Enzyme Inhibitory Effects of Daucus Virgatus (Poir.) Maire Essential oil from Algeria
Flamini, GInvestigation
;
2020-01-01
Abstract
Essential oils (EO) represent an important source of bioactive molecules and are widely used for their great efficacy relating to their different therapeutic properties. The present study aims to contribute to the valorization of Daucus virgatus (Poir.) Maire, an aromatic and native plant spontaneously growing in the North-Eastern regions of Algeria, by analyzing the chemical composition of its essential oil and evaluating the antioxidant and antimicrobial activities, together with the assay of the enzyme inhibitory effects against alpha-glucosidase and cholinesterase. Daucus virgatus EO was extracted by hydrodistillation and analyzed by gas chromatography-mass spectroscometry analysis (GC-MS). Twenty-one constituents accounting for 98% of the whole components were identified. beta-Pinene (77.9%) and alpha-pinene (7.6%) were the most abundant components. The antimicrobial activity against the pathogen microorganisms Listeria innocua, Salmonella typhimurium, Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli and Candida albicans was investigated. The best antimicrobial effect was observed against S. typhimurium and C. albicans, which showed high sensitivity, with inhibition zones ranging between 27.3 and 25 mm; the minimum inhibitory concentrations were 15.63 and 31.25 mu g ml(-1), respectively. In the DPPH test, the essential oil showed a moderate antioxidant effect, with an IC50 value of 39.61 mg ml(-1). D. virgatus EO exhibited an interesting inhibitory effect against alpha-glucosidase enzyme (IC50 = 0.35 mg ml(-1)) compared with the positive control Acarbose (IC50 = 0.24 mg ml(-1)), and a moderate inhibitory effect against cholinesterases enzymes, with IC50 values of 0.33 and 0.20 mg ml(-1) for acetylcholinesterase and butyrylcholinesterase, respectively. D. virgatus EO might be used as a promising source of natural products with potential antimicrobial, antihyperglycemic and anti-Alzheimer effects.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
