A pH stability analysis was made, in the presence of tert-butyl hydroperoxide, of both immobilized and native chloroperoxidase obtained from Caldariomyces fumago and the inactivation constants (j1) evaluated. The native enzyme displays a uni-exponential decay, whereas for the immobilized enzyme a three exponential equation describes the time dependent enzyme inactivation. For immobilized enzyme, three-exponential equation describes the enzyme time-course inactivation. The obtained inactivation constants (j3 and K3) showed an increase in the stability of a fraction of the immobilized enzyme. This is probably due to a decrease of the affinity of the enzyme for the oxidant and not to a decrease in j3 values.
|Autori:||Toti P., PETRI A., Gambicorti T., Osman A. M.; Bauer C.|
|Titolo:||Inactivation studies on native and silicagel non-homogeneous immobilized chloroperoxidase|
|Anno del prodotto:||2006|
|Digital Object Identifier (DOI):||10.1016/j.molcatb.2005.11.004|
|Appare nelle tipologie:||1.1 Articolo in rivista|