A pH stability analysis was made, in the presence of tert-butyl hydroperoxide, of both immobilized and native chloroperoxidase obtained from Caldariomyces fumago and the inactivation constants (j1) evaluated. The native enzyme displays a uni-exponential decay, whereas for the immobilized enzyme a three exponential equation describes the time dependent enzyme inactivation. For immobilized enzyme, three-exponential equation describes the enzyme time-course inactivation. The obtained inactivation constants (j3 and K3) showed an increase in the stability of a fraction of the immobilized enzyme. This is probably due to a decrease of the affinity of the enzyme for the oxidant and not to a decrease in j3 values.

Inactivation studies on native and silicagel non-homogeneous immobilized chloroperoxidase

PETRI, ANTONELLA;
2006-01-01

Abstract

A pH stability analysis was made, in the presence of tert-butyl hydroperoxide, of both immobilized and native chloroperoxidase obtained from Caldariomyces fumago and the inactivation constants (j1) evaluated. The native enzyme displays a uni-exponential decay, whereas for the immobilized enzyme a three exponential equation describes the time dependent enzyme inactivation. For immobilized enzyme, three-exponential equation describes the enzyme time-course inactivation. The obtained inactivation constants (j3 and K3) showed an increase in the stability of a fraction of the immobilized enzyme. This is probably due to a decrease of the affinity of the enzyme for the oxidant and not to a decrease in j3 values.
2006
Toti, P.; Petri, Antonella; Gambicorti, T.; Osman, A. M.; Bauer, C.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11568/106793
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