Grapevine shoot cultures infected by Grapevine Virus A (GVA) were grown on Quorin-Lepoivre (QL) medium to which ribavirin was added at 20 μg ml-1 for 180 days (6 subcoltures). Sequential assays with ELISA test during all subcoltures treatment showed that treated explants remained virus free after 60 days. Ribavirin (Rb) phytotoxicity was also observed on treated shoots that displayed a high percentage of mortality. ELISA negative explants were transferred on no added QL medium to submit to RT-PCR and nested PCR with different primers: universal and discriminating for different isolates of GVA. PCR results showed the continue presence of GVA in all selected explants and were characterized by a different response to specific primer. Rb treatment expressed a selection on the original population of isolates on mother plant producing a different composition of virus isolates, accordingly with the heterogeneous nature of GVA population documented in letterature.
Grapevine vitivirus A eradication in Vitis vinifera explants by antiviral drugs and thermotherapy
PANATTONI, ALESSANDRA;CRISTANI, CATERINA;TRIOLO, ENRICO
2007-01-01
Abstract
Grapevine shoot cultures infected by Grapevine Virus A (GVA) were grown on Quorin-Lepoivre (QL) medium to which ribavirin was added at 20 μg ml-1 for 180 days (6 subcoltures). Sequential assays with ELISA test during all subcoltures treatment showed that treated explants remained virus free after 60 days. Ribavirin (Rb) phytotoxicity was also observed on treated shoots that displayed a high percentage of mortality. ELISA negative explants were transferred on no added QL medium to submit to RT-PCR and nested PCR with different primers: universal and discriminating for different isolates of GVA. PCR results showed the continue presence of GVA in all selected explants and were characterized by a different response to specific primer. Rb treatment expressed a selection on the original population of isolates on mother plant producing a different composition of virus isolates, accordingly with the heterogeneous nature of GVA population documented in letterature.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
