Background: The ratio of two metabolites derived from nicotine smoking, 3’-hydroxycotinine (3HC) and cotinine (COT) (nicotine metabolite ratio, NMR), reflects the activity of the liver enzyme CYP2A6 and it has been shown to predict response to smoking cessation drugs in clinical trials. The aim of the present study is to facilitate translation of study findings in the real life clinical practice. Methods: We carried out a clinical validation of a mass spectrometerbased method for measuring the NMR in both plasma and saliva samples obtained from 71 smokers attending the Smoking Cessation Centre of the University Hospital of Pisa. We also performed a realtime PCR analysis for fast genotyping of CYP2A6.
Nicotine Metabolite Ratio in Smokers: A Real World Experience
Fogli S;Polini B;Carrozzi L;Pistelli F;Puppo G;Palla A;Saba A;Del Re M;
2017-01-01
Abstract
Background: The ratio of two metabolites derived from nicotine smoking, 3’-hydroxycotinine (3HC) and cotinine (COT) (nicotine metabolite ratio, NMR), reflects the activity of the liver enzyme CYP2A6 and it has been shown to predict response to smoking cessation drugs in clinical trials. The aim of the present study is to facilitate translation of study findings in the real life clinical practice. Methods: We carried out a clinical validation of a mass spectrometerbased method for measuring the NMR in both plasma and saliva samples obtained from 71 smokers attending the Smoking Cessation Centre of the University Hospital of Pisa. We also performed a realtime PCR analysis for fast genotyping of CYP2A6.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
